Jeanne C. Stachowiak

This study shows CD36 and its ligands are internalized via clathrin-mediated endocytosis, not caveolae, and disrupting clathrin inhibits ligand uptake and PROTAC-induced degradation.

This study shows that ionic strength and ionization affect membrane-tethered polymer conformation, which influences membrane curvature generation through changes in brush thickness and grafting density.

This paper uses simulations to study how protein condensates on opposite sides of a membrane interact via membrane-mediated forces, finding that curvature plays a major role in their coupling.

The paper uses simulations to show that increased lipid interdigitation mediates transbilayer coupling of protein condensates by reducing lipid entropy and altering membrane curvature.

The paper shows that balancing actin filament nucleation and elongation is necessary to deform protein condensates into high aspect ratio structures.

This study shows clathrin accessory proteins destabilize protein condensates while promoting clathrin lattice assembly and exclusion, revealing a mechanism linking phase separation to endocytosis.

This study designs intrinsically disordered ligands that control receptor uptake via endocytosis by modulating their sequence to promote or hinder internalization.

This study shows how mechanochemical feedback between confinement and actin crosslinking controls droplet shape and actin organization through dynamic relationships between bundling and deformation.

The paper demonstrates wavelength-dependent communication between synthetic cells using engineered connexin channels that respond to UV and near IR light.

The paper shows that Anillin-dependent actin assembly at PML NBs protects genome stability by shielding under-replicated DNA across cell cycles.

This study shows poly-ubiquitylated transmembrane proteins outcompete mono-ubiquitylated and non-ubiquitylated proteins for endocytic uptake by clathrin-coated vesicles.

This study shows that protein condensates and clathrin assembly regulate each other during clathrin-mediated endocytosis, enhancing vesicle formation while controlling condensate growth.

This study shows Epsin1 stabilizes initiator protein condensation during clathrin-mediated endocytosis only when ubiquitin is present, ensuring cargo-specific vesicle formation.

This study shows that transmembrane coupling of protein condensates on opposite sides of a membrane slows diffusion but accelerates growth by localizing interactions, enhancing condensate formation.

This study shows that the LIM domain of Prickle2 binds stressed actin filaments, but full-length Prickle2 does not, and identifies mutations that disrupt this interaction.

This paper develops a biophysical model of the glycocalyx's role in generating and sensing membrane curvature, showing how glycocalyx properties influence membrane shape and curvature sensing.

The paper shows that actin-binding proteins forming liquid-like condensates can polymerize and bundle actin filaments, expanding the known set of proteins capable of this function.

This paper introduces a live-cell imaging assay using TIRF microscopy to track particle uptake by clathrin-mediated endocytosis and analyzes how particle properties affect uptake efficiency.

This paper demonstrates light-activated release of molecules from synthetic cells using engineered connexon nanopores triggered by protease activity in light-sensitive liposomes.

This study shows clathrin is a strong membrane curvature sensor, with its sensitivity amplified by adaptor proteins, highlighting the role of protein networks in curvature sensing.