Li‐Chun Tu

The paper identifies RNA as the molecular glue forming a stable chromatin compartment associated with nuclear speckles, independent of speckles themselves.

CRISPR-FOIL engineers chromatin loops and compaction in live human cells using CRISPR to visualize and manipulate chromatin structure, showing its potential for gene regulation and disease therapy.

The paper uses CRISPR-Sirius to visualize differences in large-scale chromosome conformations between osteoblasts and osteosarcoma cells, revealing regulatory mechanisms involving H3K27me3 and CTCF/cohesin.

C-BERST uses dCas9-APEX2 to map proteomes near genomic loci, identifying telomere and centromere-associated proteins.

The paper uses CRISPR-Sirius to track chromosomal loci dynamics in living cells and finds cell cycle-dependent changes in DNA contortion and movement.

The paper evaluates the reliability of 3D-SPEED, a method that reconstructs spatial transport distributions in the nuclear pore complex from 2D images.