Michael Rosbash

El-INTACT nuclear purification gives clean ATAC-seq from ~120 Drosophila clock neurons and 16 PDF neurons, revealing cell-type-specific Clk enhancers; disrupting two renders flies arrhythmic.

The paper profiles circadian neuron transcriptomes across timepoints under different light conditions to reveal transcriptional and light-dependent gene regulation in Drosophila.

The paper identifies sex-specific gene expression and synaptic connections in Drosophila circadian neurons, revealing molecular mechanisms underlying sexual dimorphism in circadian behavior.

The paper shows that disrupting mitochondrial fusion in Drosophila neurons leads to a Warburg-like metabolic response that protects against neurodegeneration.

The paper identifies a conserved PER-DBT interaction critical for circadian timing and temperature compensation through structural and functional analyses in Drosophila and human cells.

This study identifies four SpsP neurons in Drosophila as an integrating hub for sleep regulation through a circuit involving upstream and downstream synaptic partners.

The paper identifies two circadian neurons in Drosophila that are influenced by light and dopamine to promote morning wakefulness.

The paper introduces FlyBox, an open-source behavior monitoring system for Drosophila that is affordable and customizable, along with FlyBoxScanner software for activity monitoring.

The paper identifies novel clock neuron subtypes in Drosophila that regulate sleep timing through distinct projection patterns and a common receptor.

The paper uses CRISPR-based methods to disrupt circadian genes in specific Drosophila neurons, revealing distinct functions of cryptochrome and PDF in circadian behavior.

The paper shows miR-190 suppresses cholinergic co-transmission in glutamatergic and GABAergic neurons by posttranscriptional regulation, affecting sleep behavior.

The paper compares TRIBE and STAMP methods for identifying RNA binding protein targets in human and Drosophila cells, finding TRIBE more effective in Drosophila.

BuTT-Seq is a new method for high-resolution profiling of transcription, showing improved efficiency and resolution over existing techniques.

The paper identifies heterogeneous clock and dopaminergic cell types in Drosophila using single-cell sequencing and shows connectivity molecules best characterize these neuron groups.

The paper identifies dopamine and GPCR-mediated modulation in Drosophila clock neurons that regulate sleep timing.

The paper introduces RiboTRIBE, a method to monitor translation by fusing ADAR to ribosomal proteins, and finds that editing occurs preferentially on polyribosome-associated transcripts.

The paper characterizes Drosophila circadian neurons using single-cell RNA sequencing, identifying 17 cell categories with time-dependent gene expression patterns.

The paper quantifies transposable element landscapes in aging Drosophila and shows RNAi mechanisms buffer TE expansion despite increased TE expression.

This study shows how food deprivation alters olfactory behavior in Drosophila larvae by changing neural processing in the antennal lobe through state-dependent circuit dynamics.

The paper identifies 4E-BP mRNA targets in Drosophila and mammals using hyperTRIBE, showing they have specific motifs and are enriched in translation-related pathways.

This study identifies CREB signaling as a pathway inhibited by TDP-43 dysfunction, leading to reduced dendritic complexity in neurodegenerative diseases.

The paper demonstrates that FlyBox, a tracking system originally for Drosophila, can effectively measure locomotor activity in Anopheles and Aedes mosquitoes over multiple days under different light conditions.

The paper shows that network communication is important for Drosophila circadian rhythms, with specific neuron groups relying on it for period determination.

The paper identifies a light-mediated pathway in Drosophila that uses PDF-releasing lLNv neurons to adjust circadian rhythms to long day conditions.

The paper identifies a novel AstC/AstC-R2 signaling pathway in Drosophila that modulates evening locomotor activity under short photoperiods.

This paper identifies a circadian output circuit in Drosophila that regulates sleep-wake arousal threshold by connecting circadian neurons to homeostatic sleep centers.

This paper introduces HyperTRIBE, an improved version of TRIBE that enhances RNA editing efficiency, leading to more accurate identification of RNA-binding protein targets.