Richard G. James

Antibody-dependent priming of spontaneous germinal centers by autoreactive B cells

This study shows autoreactive B cells can initiate spontaneous germinal centers and autoimmunity through antibody-dependent mechanisms, independent of T cell interactions or antigen presentation.

An integrated, scaled approach to resolve TSC2 variants of uncertain significance

Abundance scores for 8,891 TSC2 missense variants, plus mTOR pathway activity scores for 391, were calibrated to ACMG/AMP evidence. They putatively reclassify 212 of 276 clinical-cohort missense VUS.

A scalable approach to resolving variants of uncertain significance

The paper develops a scalable workflow to reclassify variants of uncertain significance using experimental and predictive evidence, resolving 75% of VUS in 40 genes.

Humanized mice enable in vivo evaluation of engineered plasma cell biology and therapeutic function

This study develops a humanized mouse model to evaluate engineered plasma cells and their therapeutic antibody secretion, showing long-term antibody production and neutralization of SARS-CoV-2.

Sealable capped nanovials for high-throughput screening of cell growth and function

This paper introduces capped nanovials as a scalable platform for single-cell and small colony culture, enabling high-throughput screening of growth and function with improved signal-to-noise ratios and co-culture capabilities.

Linking single-cell transcriptomes with secretion using secretion-encoded single-cell sequencing (SEC-seq)

SEC-seq links single-cell transcriptomes with secretion levels by isolating cells and secretions in Nanovials, enabling analysis of secretory phenotypes and their genetic drivers.

Multi-reactive hydrogel nanovials for temporal control of secretion capture from antibody-secreting cells

This paper introduces multi-reactive hydrogel nanovials that enable temporal control of secretion capture from antibody-secreting cells, achieving high-purity isolation of antigen-specific hybridomas.