Craig R. Roy

This study uses cryo-EM to reveal the structural dynamics and activation mechanism of the Legionella Dot/Icm T4SS, showing how it transitions from a resting state to an active secretion machine.

This paper identifies BinA, a Legionella effector that rewires amino acid transport to sensitize mTORC1 signaling in macrophages, enhancing bacterial replication.

This paper uses FIB-SEM and machine learning to study Coxiella burnetii infection, revealing 3D structure variations in bacteria and CCVs and showing that a mutant's vacuole defect does not affect its developmental cycle.

This study shows that Type IV Secretion Systems (T4SSs) prime for substrate delivery through contact-induced lipid mixing, not requiring ATP or full complex assembly, and this mechanism applies to both Dot/Icm and RK2 T4SSs.

This study uses cryo-electron microscopy to reveal the structure of the Dot/Icm T4SS in Legionella pneumophila, identifying the DotA-IcmX complex as the gatekeeper for effector translocation.

This study investigates the secretion mechanism of transmembrane effector proteins by Legionella T4bSS, revealing a two-step process involving an inner membrane intermediate.