Michael E. Pyne

The paper repurposes the yeast Ehrlich pathway to synthesize pharmaceutical alkaloids using retrobiosynthetic analysis and enzyme screening.

CRAPS enables in vivo assembly of multiple pathway configurations by using yeast chromosomal repair and gRNA activation, resulting in diverse carotenoid-producing strains.

This paper improves the microbial synthesis of benzylisoquinoline alkaloids in yeast by using human monoamine oxidase A to increase selectivity and yield of (S)-reticuline and its derivatives.

The paper screens non-conventional yeasts for organic acid tolerance and engineers Pichia occidentalis to produce cis,cis-muconic acid with improved metrics compared to Saccharomyces cerevisiae.

This study elucidates the biosynthesis of unique benzylisoquinoline alkaloids in sacred lotus and enables their microbial synthesis.

This paper describes a yeast platform that significantly increases the production of tetrahydroisoquinoline alkaloids, enabling the synthesis of natural and unnatural derivatives.