The paper identifies brain-peripheral DNA methylation sites (BrESS) that consistently predict brain methylation across tissues, showing they are not linked to disease heritability.

bioRxiv · Oct 24, 2026 · PreprintNeuroscience

Zuranolone mitigates delirium-like bispectral EEG changes, behavioral deficits, and neuroinflammation across surgical and inflammatory mouse models and age groups

Bun Aoyama, Shota Nishitani, Kyosuke Yamanishi, Hieu Dinh Nguyen, Rika Sakuma, Takaya Ishii, Yukiko Ikeda, Tsuyoshi Nishiguchi, Ilgin Genc, Nathan James Phuong, Nipun Gorantla, Tomoteru Seki, Akiyoshi Shimura, Takashi Kawano, Gen Shinozaki

bioRxiv · May 21, 2026 · PreprintNeuroscience

Non-linear Age-related Change in Human Interleukin-11 and the receptor subunit alpha DNA Methylation

Akiyoshi Shimura, Varun Bhamidipati Dwaraka, Kyosuke Yamanishi, Tomoteru Seki, Tsuyoshi Nishiguchi, Bun Aoyama, Takaya Ishii, Nathan James Phuong, Nipun Gorantla, Hieu Dinh Nguyen, Therese Anne Santiago, Shota Nishitani, Ryan C. Smith, Gen Shinozaki

The paper examines age-related DNA methylation changes in IL-11 and IL-11RA genes, finding inverse U-shaped patterns that suggest non-linear regulation of IL-11 expression and sensitivity across life stages.

Biochemistry and Biophysics Reports · Mar 19, 2026Peer-reviewedGenetics

Linking Pain and Delirium via Microglial Activation: A Mouse Study Using BSEEG, Behavioral Assays, Immunohistochemistry, and RNA Sequencing

Kyosuke Yamanishi, Riley Merkel, Nathan James Phuong, Victoria Anderson, Shota Nishitani, Tsuyoshi Nishiguchi, Takaya Ishii, Bun Aoyama, Nipun Gorantla, Hieu Dinh Nguyen, Ilgin Genc, Hisato Matsunaga, Vivianne L. Tawfik, Gen Shinozaki

This study links neuropathic pain and delirium via microglial activation in mice using combined behavioral, electrophysiological, and transcriptomic analyses.

bioRxiv · Oct 29, 2025 · PreprintNeuroscience

The age-related susceptibility to postoperative delirium quantified by bispectral electroencephalography (BSEEG) correlates with postoperative delirium-like behavior in mice

Tsuyoshi Nishiguchi, Kyosuke Yamanishi, Akiyoshi Shimura, Tomoteru Seki, Takaya Ishii, Bun Aoyama, Nipun Gorantla, Hieu Dinh Nguyen, Nathan James Phuong, Takehiko Yamanashi, Masaaki Iwata, Gen Shinozaki

This study correlates BSEEG scores with delirium-like behavior in mice, showing age-related differences in the relationship between BSEEG and behavioral outcomes.

Psychiatry and Clinical Neurosciences Reports · Jul 6, 2025Peer-reviewedAnimal Behavior & Cognition

Glial Contribution to the Pathogenesis of Post-Operative Delirium Revealed by Multi-omic Analysis of Brain Tissue from Neurosurgery Patients

Takaya Ishii, Tao Wang, Kazuki Shibata, Shota Nishitani, Takehiko Yamanashi, Nadia E. Wahba, Tomoteru Seki, Kaitlyn J. Thompson, Kyosuke Yamanishi, Tsuyoshi Nishiguchi, Akiyoshi Shimura, Bun Aoyama, Nipun Gorantla, Nathan James Phuong, Hieu Dinh Nguyen, , Gen Shinozaki

This study uses multi-omic analysis of brain tissue from POD patients to show glial cell contributions to pathogenesis, identifying epigenetic and transcriptomic changes in microglia and astrocytes.

bioRxiv · Mar 13, 2025 · PreprintNeuroscience