The paper examines age-related DNA methylation changes in IL-11 and IL-11RA genes, finding inverse U-shaped patterns that suggest non-linear regulation of IL-11 expression and sensitivity across life stages.

Biochemistry and Biophysics Reports · Mar 19, 2026Peer-reviewedGenetics

Linking Pain and Delirium via Microglial Activation: A Mouse Study Using BSEEG, Behavioral Assays, Immunohistochemistry, and RNA Sequencing

Kyosuke Yamanishi, Riley Merkel, Nathan James Phuong, Victoria Anderson, Shota Nishitani, Tsuyoshi Nishiguchi, Takaya Ishii, Bun Aoyama, Nipun Gorantla, Hieu Dinh Nguyen, Ilgin Genc, Hisato Matsunaga, Vivianne L. Tawfik, Gen Shinozaki

This study links neuropathic pain and delirium via microglial activation in mice using combined behavioral, electrophysiological, and transcriptomic analyses.

bioRxiv · Oct 29, 2025 · PreprintNeuroscience

Epigenetic markers of middle-age: non-linear DNA methylation changes with aging in humans

Akiyoshi Shimura, Kyosuke Yamanishi, Takaya Ishii, Tomoteru Seki, Tsuyoshi Nishiguchi, Bun Aoyama, Therese Santiago, Varun Bhamidipati Dwaraka, Ryan C. Smith, Gen Shinozaki

The paper identifies non-linear DNA methylation patterns with aging, showing peaks or troughs in middle age and linking them to disease onset.

bioRxiv · Aug 14, 2025 · PreprintGenomics

The age-related susceptibility to postoperative delirium quantified by bispectral electroencephalography (BSEEG) correlates with postoperative delirium-like behavior in mice

Tsuyoshi Nishiguchi, Kyosuke Yamanishi, Akiyoshi Shimura, Tomoteru Seki, Takaya Ishii, Bun Aoyama, Nipun Gorantla, Hieu Dinh Nguyen, Nathan James Phuong, Takehiko Yamanashi, Masaaki Iwata, Gen Shinozaki

This study correlates BSEEG scores with delirium-like behavior in mice, showing age-related differences in the relationship between BSEEG and behavioral outcomes.

Psychiatry and Clinical Neurosciences Reports · Jul 6, 2025Peer-reviewedAnimal Behavior & Cognition

Glial Contribution to the Pathogenesis of Post-Operative Delirium Revealed by Multi-omic Analysis of Brain Tissue from Neurosurgery Patients

Takaya Ishii, Tao Wang, Kazuki Shibata, Shota Nishitani, Takehiko Yamanashi, Nadia E. Wahba, Tomoteru Seki, Kaitlyn J. Thompson, Kyosuke Yamanishi, Tsuyoshi Nishiguchi, Akiyoshi Shimura, Bun Aoyama, Nipun Gorantla, Nathan James Phuong, Hieu Dinh Nguyen, , Gen Shinozaki

This study uses multi-omic analysis of brain tissue from POD patients to show glial cell contributions to pathogenesis, identifying epigenetic and transcriptomic changes in microglia and astrocytes.

bioRxiv · Mar 13, 2025 · PreprintNeuroscience

LPS-induced delirium-like behavior and microglial activation in mice correlate with bispectral electroencephalography (BSEEG)

Tsuyoshi Nishiguchi, Kyosuke Yamanishi, Nipun Gorantla, Akiyoshi Shimura, Tomoteru Seki, Takaya Ishii, Bun Aoyama, Johnny R. Malicoat, Nathan James Phuong, Nicole Jade Dye, Takehiko Yamanashi, Masaaki Iwata, Gen Shinozaki

This study validates the use of BSEEG in an LPS-induced mouse model of delirium, showing its correlation with behavioral changes and microglial activation.

The Journals of Gerontology Series A · Nov 2, 2024Peer-reviewedNeuroscience

The bispectral EEG (BSEEG) method quantifies post-operative delirium-like states in young and aged mice after head mount implantation surgery

Tsuyoshi Nishiguchi, Kazuki Shibata, Kyosuke Yamanishi, Mia Nicole Dittrich, Noah Yuki Islam, Shivani S. Patel, Nathan James Phuong, Pedro S. Marra, Johnny R. Malicoat, Tomoteru Seki, Yoshitaka Nishizawa, Takehiko Yamanashi, Masaaki Iwata, Gen Shinozaki

This study validates the BSEEG method for quantifying post-operative delirium-like states in mice, showing age-related differences in recovery.

The Journals of Gerontology Series A · Jun 15, 2024Peer-reviewedNeuroscience