Author

Joshua B. Krueger

University of MinnesotaORCID 0009-0002-7658-149XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Bioengineering· 2 Immunology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Approach:genetic perturbation / CRISPR· 2 Research Focus:host-pathogen & immune response· 2 Organism:human· 2 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Research Focus:gene regulatory network inference· 1

Frequent Co-Authors

Branden S. MoriarityiD· 3 shared Beau R. WebberiD· 2 shared Bibekananda KariD· 2 shared Bryce J. WickiD· 2 shared Erin M. Stelljes· 2 shared Joseph G. SkeateiD· 2 shared Mitchell G. KluesneriD· 2 shared Alexandria K. Gilkey· 1 shared

Activity by Year

2026-2026 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 43 mutual co-author links

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3 shared papersBioengineering

Branden S. MoriarityORCID

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3 papers

Recent·Most cited

A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells

Joseph G. Skeate, Nicholas J. Slipek, Walker S. Lahr, Shambojit Roy, Bryce J. Wick, Bibekananda Kar, Jae-Woong Chang, Erin M. Stelljes, Alexandria K. Gilkey, Prateek P. Thenge, Miechaleen D. Diers, Joshua B. Krueger, Ethan M. Niemeyer, Cara-lin Lonetree, Mitchell G. Kluesner, , Beau R. Webber

This paper develops a base editing platform to efficiently and safely engineer CAR T cells with multiple gene knockouts and insertions.

Molecular Therapy · Jul 18, 2026Peer-reviewedBioengineering

Multiplex engineering of rhesus macaque NK cells enhances homing to sites of HIV replication in B cell follicles.

Liliana Kopeck Thron, Mary S. Pampusch, Jae‐Woong Chang, Joshua B. Krueger, Maxwell E. Cantor, Matthew J Johnson, Dawn M. Dudley, Branden S. Moriarity, Pamela J. Skinner

This study develops and tests multiplex-engineered NK cells in rhesus macaques to target HIV reservoirs by enhancing homing to B cell follicles.

bioRxiv · Jun 11, 2026 · PreprintBioengineering

Efficient multiplex non-viral engineering and expansion of polyclonal γδ CAR-T cells for immunotherapy

Jacob E. Bridge, Matthew J. Johnson, Bibekananda Kar, Jihyun Kim, Sophia J. Wenthe, Joshua B. Krueger, Bryce J. Wick, Mitchell G. Kluesner, Andrew T. Crane, Jason Bell, Lage von Dissen, Erin M. Stelljes, Joseph G. Skeate, Branden S. Moriarity, Beau R. Webber

This paper develops a scalable, non-viral method to engineer and expand polyclonal γδ CAR-T cells, showing their functional efficacy and subset-specific responses in vivo.

Molecular Therapy · Mar 11, 2026Peer-reviewedImmunology