Malcolm L. Wells

Bidirectional allosteric ligand regulation in a central glycolytic enzyme

This study resolves a long-standing discrepancy between the structural and functional properties of PFK by showing how activator and inhibitor binding differentially reweights its conformational ensemble.

Distinct energetic blueprints diversify function of conserved protein folds

The paper decodes energetic blueprints of conserved protein folds to explain functional diversity and enables engineered transcription factors with ligand-driven responses.

Simultaneous single-cell profiling of the transcriptome and proteome

This paper describes a method to simultaneously profile the transcriptome and proteome from single cells, showing moderate correlation between the two modalities.

A Hotspot Phosphorylation Site on SHP2 Drives Oncoprotein Activation and Drug Resistance

This paper identifies phosphorylation of SHP2 at tyrosine 62 as a mechanism of resistance to SHP2 inhibitors and a new drug target.

Site-resolved energetic information from HX/MS experiments

This paper introduces PIGEON-FEATHER, a method to calculate free energies of protein conformational ensembles from HX/MS data, revealing evolutionary differences in enzyme inhibitors and ligand-induced reweighting in a protein-DNA complex.