Nature CommunicationsMolecular BiologyPeer-reviewed journal article
A genetically encoded fluorescent biosensor for extracellular L-lactate
Yusuke NasuiD, Ciaran Murphy‐RoyaliD, Yurong WeniD, Jordan N. Haidey, Rosana S. MolinaiD, Abhi AggarwaliD, Shuce ZhangiD, Yuki KamijoiD, Marie-Ève PaquetiD, Kaspar PodgorskiiD, Mikhail A. DrobizheviD, Jaideep Singh BainsiD, M. Joanne LemieuxiD, Grant Robert GordoniD, Robert E. CampbelliD
Abstract
To enable investigations of the emerging roles of cell-to-cell shuttling of L-lactate, we have developed an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. We demonstrate that this biosensor, designated eLACCO1.1, enables minimally invasive cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
- Citations
- 110
- FWCI
- 7.35
- Field percentile
- top 1%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A red fluorescent genetically encoded biosensor for extracellular L-lactate
Y. Nasu, Y. Kamijo, … R. E. Campbell·bioRxiv·Aug 30, 2022
- Improved genetically encoded fluorescent biosensors for monitoring of intra- and extracellular L-lactate
Yusuke Nasu, Abhi Aggarwal, … Robert E. Campbell·bioRxiv·Dec 27, 2022
- High performance genetically-encoded green fluorescent biosensors for intracellular L-lactate
G. N. T. Le, S. Hario, … R. E. Campbell·bioRxiv·Oct 19, 2022
- Calcium-independent biosensors for multiplexed in vivo imaging of extracellular lactate dynamics
Jia-Jin Liu, Hidenobu Mizuno, … Yusuke Nasu·bioRxiv·Oct 5, 2026
- An engineered lactate responding promoter system operating in glucose-rich and anaerobic environments.
Ana Zúñiga, Hung‐Ju Chang, … Jerome Bonnet·bioRxiv·Jan 6, 2021
- Highly responsive single-fluorophoreindicator to explore lactate dynamics in high calcium environments
A. Galaz, P. Y. Sandoval, … A. San Martin·bioRxiv·Oct 1, 2020