Mikhail A. Drobizhev

Calcium-independent biosensors for multiplexed in vivo imaging of extracellular lactate dynamics

The paper develops Ca2+-independent lactate biosensors for multiplexed in vivo imaging of extracellular lactate dynamics alongside neuronal activity.

ECgo: All-Optical Induction of Single Endothelial Cell Injury and Capillary Occlusion in the Brain

The paper introduces ECgo, an optical method to induce single endothelial cell injury and capillary occlusion in the mouse brain with high spatial precision, enabling studies of capillary repair and dysfunction.

hpGRISZ: a high-performance fluorescent biosensor for in vivo imaging of synaptic zinc dynamics

The paper introduces hpGRISZ, a fluorescent biosensor for in vivo imaging of synaptic zinc dynamics, showing high responsiveness and suitability for detecting extracellular zinc release.

Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions

This study develops two red genetically encoded potassium indicators (RGEPOs) for real-time imaging of intracellular and extracellular potassium dynamics in various biological systems.

High-performance genetically-encoded green and red fluorescent biosensors for pyruvate

The paper develops high-performance green and red fluorescent biosensors for pyruvate with large fluorescence intensity changes and varying affinities, enabling multicolor imaging in mammalian cells.

Far-red fluorescent genetically encoded calcium ion indicators

This paper introduces two new far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, characterized for their Ca2+ sensitivity and performance in neuronal imaging.

Directionality of two-photon absorption in representative fluorescent proteins

The paper measures two-photon absorptivity tensors of fluorescent proteins to understand their directionality and provides insights for biosensor development.

A blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption

The paper develops T-GECO1, a blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon brightness for improved neuronal activity imaging.

A Genetically Encoded Far-Red Fluorescent Indicator for Imaging Synaptically-Released Zn2+

This study develops a genetically encoded far-red fluorescent indicator (FRISZ) to image synaptic Zn2+ dynamics in live animals and demonstrates its use in the auditory cortex of awake mice.

Improved genetically encoded fluorescent biosensors for monitoring of intra- and extracellular L-lactate

This paper develops improved fluorescent biosensors for monitoring extracellular and intracellular L-lactate in mammalian cells, showing enhanced sensitivity and response kinetics.

Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells

This paper describes a method for rapidly optimizing fluorescent proteins in mammalian cells, resulting in enhanced near-infrared fluorescent proteins with improved intracellular brightness and multicolor imaging capabilities.

A genetically encoded fluorescent biosensor for extracellular L-lactate

This paper develops a genetically encoded fluorescent biosensor for extracellular L-lactate and demonstrates its use in imaging in cultured mammalian cells and brain tissue.

Voltage imaging in Drosophila using a hybrid chemical-genetic rhodamine voltage reporter

This paper develops a hybrid chemical-genetic voltage reporter for voltage imaging in Drosophila, enabling selective neuron labeling and voltage monitoring in intact tissues.

A Fast High-Affinity Fluorescent Serotonin Biosensor Engineered from a Tick Lipocalin

The authors retracted their paper due to irreproducible results and contamination issues in their experimental materials.

High throughput instrument to screen fluorescent proteins under two-photon excitation

The paper introduces a high-throughput instrument called GIZMO to screen fluorescent protein mutants under two-photon excitation, enabling efficient evolution of proteins optimized for this excitation method.