Journal of Structural BiologyMolecular BiologyPeer-reviewed journal article
Multishot Tomography for High-Resolution In Situ Subtomogram Averaging.
Sagar KhavnekariD, William WaniD, Parijat MajumderiD, Wojciech WietrzyñskiiD, Philipp S. ErdmanniD, Jürgen M. PlitzkoiD
Abstract
Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, and when combined with focused ion beam (FIB) milling, macromolecules can be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET can be slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic acquisition scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, which yields sub-nanometer in situ STA averages.
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