Nature BiotechnologyBioinformaticsPeer-reviewed journal article
Analysis and comparison of genome editing using CRISPResso2
Kendell ClementiD, Holly A. ReesiD, Matthew C. CanveriD, Jason M. Gehrke, Rick FarouniiD, Jonathan Y. HsuiD, Mitchel A. ColeiD, David R. LiuiD, J. Keith JoungiD, Daniel E. BaueriD, Luca PinelloiD
Abstract
Genome editing technologies are rapidly evolving, and analysis of deep sequencing data from target or off-target regions is necessary for measuring editing efficiency and evaluating safety. However, no software exists to analyze base editors, perform allele-specific quantification or that incorporates biologically-informed and scalable alignment approaches. Here, we present CRISPResso2 to fill this gap and illustrate its functionality by experimentally measuring and analyzing the editing properties of six genome editing agents.
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