bioRxivMolecular BiologyPreprint
RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells
Peiguo ShiiD, Michael Robert MurphyiD, Alexis O. AparicioiD, Jordan S. KesneriD, Zhou Fang, Ziheng CheniD, Aditi Trehan, Xuebing WuiD
Abstract
While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.
- organism: human
- assay: bulk RNA-seq
- research focus: perturbation response prediction
- approach: experimental assay
- Citations
- 21
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