Author

P. Lotfy

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Biochemistry· 1 Immunology· 1

Signature Assays, Organisms & Methods

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Research Focus:gene regulatory network inference· 3 Organism:human· 3 Approach:deep learning· 2 Approach:statistical model· 2 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1 Research Focus:differential expression· 1 Assay:DNA methylation· 1

Frequent Co-Authors

P. D. Hsu· 3 shared M. G. Durrant· 2 shared S. Konermann· 2 shared A. Fanton· 1 shared A. H. Owings· 1 shared A. K. Shalek· 1 shared A. Parker· 1 shared A. S. Bhatt· 1 shared

Activity by Year

2018-2021 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 18 mutual co-author links

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3 shared papersBioengineering

P. D. Hsu

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Large-scale discovery of recombinases for integrating DNA into the human genome

M. G. Durrant, A. Fanton, J. Tycko, M. Hinks, S. S. Chandrasekaran, N. T. Perry, J. Schaepe, P. P. Du, P. Lotfy, M. C. Bassik, L. Bintu, A. S. Bhatt, P. D. Hsu

This paper identifies and validates new large serine recombinases (LSRs) for efficient DNA integration into human genomes, achieving higher efficiency than existing tools.

bioRxiv · Nov 5, 2021Bioengineering

Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting

J. Wei, P. Lotfy, K. Faizi, S. Baungaard, E. Gibson, E. Wang, H. Slabodkin, E. Kinnaman, S. Chandrasekaran, H. Kitano, M. G. Durrant, C. V. Duffy, P. D. Hsu, S. Konermann

The paper develops a deep learning model to predict CRISPR-Cas13d guide RNA efficiency and identifies novel orthologs with improved specificity and reduced toxicity for RNA targeting.

bioRxiv · Sep 14, 2021Bioengineering

Impaired local intrinsic immunity to SARS-CoV-2 infection in severe COVID-19

C. G. K. Ziegler, V. N. Miao, A. H. Owings, A. W. Navia, Y. Tang, J. D. Bromley, P. Lotfy, M. Sloan, H. Laird, H. B. Williams, M. George, R. S. Drake, T. Christian, A. Parker, C. B. Sindel, , J. Ordovas-Montanes

This study analyzes nasopharyngeal samples from COVID-19 patients and healthy controls to identify how SARS-CoV-2 infection affects respiratory epithelial cells and immune responses, finding impaired local intrinsic immunity in severe cases.

bioRxiv · Feb 20, 2021Immunology

Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d

C. Zhang, S. Konermann, N. J. Brideau, P. Lotfy, S. J. Novick, T. Strutzenberg, P. R. Griffin, P. D. Hsu, D. Lyumkis

The paper determines the structural basis of CRISPR-Cas13d's RNA-guided ribonuclease activity through cryo-EM and biochemical analysis, revealing its molecular architecture and activation mechanisms.

bioRxiv · May 4, 2018Biochemistry