bioRxivCell BiologyPreprint
Resolution of Reprogramming Transition States by Single Cell RNA-Sequencing
Lin Guo, Xiaoshan Wang, Mingwei Gao, Lihui LiniD, Junqi KuangiD, Yuanbang Mai, Fang Wu, He Liu, Jiaqi Yang, Shilong Chu, Hong SongiD, Yujian Liu, Jiadong Liu, Jinyong Wang, Guangjin PaniD, Andrew P. HutchinsiD, Jing Liu, Jiekai CheniD, Duanqing PeiiD
Abstract
The Yamanaka factors convert mouse embryonic fibroblasts (MEFs) into induced pluripotent stem cells (iPSCs) through a highly heterogeneous process. Here we profile single cells undergoing an optimized 7-day reprogramming process and show that cells start reprogramming relatively in sync, but diverge into two branches around day 2. The first branch of cells expressing Cd34/Fxyd5/Psca become nonpluripotent. The second one contains cells that are first Oct4+, then Dppa5a+ and pluripotent. We show that IFN-{gamma} blocks this late transition. Our results reveal the heterogeneous nature of somatic cell reprogramming, identify Dppa5a as a marker for pluripotent and innate immunity as a potential barrier for reprogramming.\n\nOne Sentence SummarySingle cell RNA sequencing reveals a continuum of cell fates from somatic to pluripotent and Dppa5a as a marker for chimera-competent iPSCs.
- organism: mouse
- assay: scRNA-seq
- research focus: cell type annotation
- research focus: clustering
- research focus: differential expression
- research focus: cell-cell communication
- approach: experimental assay
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