Nature NeuroscienceDevelopmental BiologyPeer-reviewed journal article
Regulation of Cell-Type-Specific Transcriptomes by miRNA Networks During Human Brain Development
Tomasz Jan NowakowskiiD, Neha RaniiD, Mahdi GolkaramiD, Hongjun ZhouiD, Beatriz AlvaradoiD, Kylie Huch, Jay A. A. WestiD, Anne A. Leyrat, Alex A. PolleniD, Arnold R. KriegsteiniD, Linda Ruth PetzoldiD, Kenneth S. KosikiD
Abstract
MicroRNAs (miRNAs) regulate many cellular events by regulating hundreds of mRNA transcripts. However, it is unclear how miRNA-mRNA interactions are contextualized into the framework of transcriptional heterogeneity among closely related cells of the developing human brain. By combining the multiple complementary approaches, AGO2-HITS-CLIP, single-cell profiling and bipartite network analysis, we show that the miRNA-mRNA network operates as functional modules related to cell-type identities and undergo dynamic transitions during brain development.
- organism: human
- assay: bulk RNA-seq
- assay: spatial transcriptomics
- research focus: gene regulatory network inference
- research focus: clustering
- research focus: differential expression
- approach: statistical model
- approach: experimental assay
- Citations
- 166
- FWCI
- 5.05
- Field percentile
- top 2%
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