bioRxivMicrobiologyPreprint
Gain-of-function assay for SARS-CoV-2 Mpro inhibition in living cells
S. A. Moghadasi, J. T. Becker, C. Belica, C. Wick, W. L. Brown, R. Harris
Abstract
The main protease, Mpro, of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for Mpro function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.
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