An Enzyme-Linked Immunosorbent Assay (ELISA)-based Activity Assay for AMP-Activated Protein Kinase (AMPK)

AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism as well as other cellular processes. Efforts have been made to discover pharmacological modulators of AMPK activity because of their therapeutic potentials against various diseases. Measuring AMPK activity in vitro is a fundamental step for testing AMPK activators and inhibitors. Here we report an enzyme-linked immunosorbent assay (ELISA)-based AMPK activity assay with simple steps and high sensitivity, which represents an alternative in-house method to the traditional radioactive method as well as other techniques that require special reagents and commercial reagent kits.