Wim J. H. Hagen

This paper introduces a crossed laser phase plate (xLPP) for cryoEM, demonstrating improved contrast and resolution in imaging biological samples.

The paper uses cryo-CLEM to visualize and analyze the ultrastructural organization of mitotic chromosomes, identifying non-histone macromolecular densities and surface particle clusters.

The paper develops a method to measure and control ice thickness in the VitroJet for cryo-EM, improving efficiency in single particle structure determination.

The paper uses AI to create a dynamic model of the human nuclear pore complex, showing how the protein scaffold interacts with the nuclear envelope.

The paper introduces an energy filter-based plasmon imaging method to efficiently screen gold grids in cryo-EM for optimal ice thickness.

The paper evaluates the performance of new cryo-EM hardware for cryo-ET and subtomogram averaging using EIAV virus-like particles, showing comparable resolution and improved reconstructions with advanced processing methods.

This study uses cryo electron tomography and simulations to analyze the structure of SARS-CoV-2 spike protein in situ, revealing three hinges in the stalk domain that enable orientational flexibility.

The paper determines the in-cell architecture of a transcribing and translating expressome in Mycoplasma pneumoniae using integrative structural methods.

The paper presents a cryo-EM structure of yeast Maf1 bound to Pol III, revealing how Maf1 represses transcription by sequestering initiation elements and blocking the active site.

This study benchmarks tomographic acquisition schemes for cryo electron tomography to optimize resolution for subtomogram averaging using HIV-1 lattice.

This study determines the structural basis of p62/SQSTM1 PB1 domain filaments, their role in phase-separated p62 bodies, and their importance in cargo recognition and lysosomal targeting.