bioRxivNeurosciencePreprint
GPX4 Promotes Optic Nerve Regeneration and RGC Neuroprotection
Ming YangiD, Fuyun Bian, Xue FengiD, Liang LiiD, Haoliang HuangiD, Liang LiuiD, Roopa DalaliD, Hang YangiD, Pranav Varma SuraparajuiD, Frank Cao, Petrina Ong, Alexandria Luo, Dong LiuiD, Yang HuiD
Abstract
Preventing retinal ganglion cells (RGCs)s soma and axon degeneration and promoting optic nerve (ON) regeneration holds great promise for effective glaucoma treatment. To explore potential neural repair strategies, we focused on glutathione peroxidase 4 (GPX4), a key regulator of lipid peroxidation. GPX4 is upregulated in surviving RGCs after acute ON crush or chronic ocular hypertension insult, and also in regenerating RGCs. AAV-mediated RGC-specific overexpression of GPX4 promotes significant ON regeneration and RGC survival, along with visual functional preservation, demonstrating the detrimental role of lipid peroxidation in glaucoma and the therapeutic potential of modulating lipid peroxidation through GPX4 in optic neuropathies.
- Citations
- 2
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- RGC-specific reversal of lipid peroxidation drives neuroprotection and vision restoration in optic nerve ischemia by targeting GPX4
Ming Yang, Jie Pan, … Yaping Joyce Liao·bioRxiv·Aug 29, 2026
- Protective Effects of Lipoxin A4 and B4 Signaling on the Inner Retina in a Mouse Model of Experimental Glaucoma
Hsin-Hua Liu, Paul Francis Cullen, … John G. Flanagan·bioRxiv·Jan 17, 2024
- Combined inhibition of apoptosis and necrosis promotes transient neuroprotection of retinal ganglion cells and partial axon regeneration after optic nerve damage
Maki Kayama, Kumiko Omura, … Demetrios G. Vavvas·bioRxiv·Jun 27, 2018
- The 5-HT1A receptor antagonist WAY-100635 maleate reprograms metabolism to promote RGC differentiation and regeneration in retino-visual centers
Sayanta Dutta, Michelle L. Surma, … Arupratan Das·Communications MedicinePeer-reviewed·Apr 2, 2026
- Overlapping transcriptional programs promote survival and axonal regeneration of injured retinal ganglion cells
Anne Jacobi, Nicholas M. Tran, … Joshua R. Sanes·NeuronPeer-reviewed·Jun 28, 2022
- AKAP1 regulates mitochondrial and synaptic homeostasis to enable neuroprotection and repair in retinal ganglion cell degeneration
Tonking Bastola, Keun-Young Kim, … Won‐Kyu Ju·bioRxiv·Nov 24, 2025