Nature CommunicationsNeurosciencePeer-reviewed journal article
A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo
Takaki YahiroiD, Landon Bayless-EdwardsiD, James A. Jones, Yizhou ZhuoiD, Lei MaiD, Maozhen Qin, Tianyi MaoiD, Haining ZhongiD
Abstract
We report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Forster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enables in vivo imaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.
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- top 33%
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