Haining Zhong

SynAPSeg provides a framework and dataset for deep learning-based synapse detection and quantification, achieving expert-level performance and revealing age-related synaptic changes.

The paper introduces AKAR6, a new suite of FRET-based PKA sensors with enhanced sensitivity and selectivity for detecting compartment-specific PKA activity in live cells.

The paper introduces a genetically encoded fluorescence sensor for PKC activity, demonstrating its use in vivo and identifying PKC activity in specific neurons during locomotion.

This study shows that cerebellar climbing fibers encode perceptual choices during decision-making in mice, with their activity reshaped by learning and critical for discrimination.

The paper develops methods to image glutamatergic synapses in hippocampal tissue using cryo-electron tomography, enabling high-resolution visualization of synaptic structures.

The paper demonstrates that glutamate and GABA are co-released by neurons in multiple brain regions, as evidenced by biphasic miniature postsynaptic currents observed in striatal neurons and other areas.

This study identifies Btbd11 as an inhibitory interneuron-specific protein that supports glutamatergic synapse structure and function, with implications for neural circuitry and behavior.

The paper develops improved genetically-encoded cAMP sensors, cAMPFIREs, for high-sensitivity in vivo imaging of cAMP dynamics in neurons and Drosophila.

This paper develops a new genetically encoded fluorescent cAMP indicator, G-Flamp1, which shows high sensitivity, brightness, and fast response kinetics for in vivo cAMP imaging.

The paper uses in vivo imaging to compare synaptic dynamics between excitatory synapses on pyramidal neurons and inhibitory interneurons, finding that PV+ synapses are more stable and less heterogeneous.