Cell stem cellDevelopmental BiologyPeer-reviewed journal article
Bovine blastocyst like structures derived from stem cell cultures
Carlos A. Pinzón-ArteagaiD, Yinjuan WangiD, Yulei WeiiD, Ana Elisa Ribeiro OrsiiD, Leijie LiiD, Giovanna Nascimento ScatoliniD, Lizhong LiuiD, Masahiro SakuraiiD, Jianfeng YeiD, Hao MingiD, Leqian YuiD, Bo LiiD, Zongliang JiangiD, Jun WuiD
Abstract
Understanding blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. We developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via the assembly of trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, and represent an accessible in vitro model for studying bovine embryogenesis.
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