Zongliang Jiang

A Universal 6iL/E4 Culture System for Deriving and Maintaining Embryonic Stem Cells Across Mammalian Species

The paper develops a serum-free 6iL/E4 culture system that enables derivation and maintenance of embryonic stem cells (ESCs) across multiple mammalian species, revealing conserved mechanisms for ESC self-renewal.

Tetrahydrocannabinol exposure to postejaculatory sperm compromises sperm structure, function, the epigenome, and early embryo development

This study shows that THC exposure to postejaculatory sperm reduces motility, damages DNA, impairs acrosome integrity, alters the methylome, and decreases embryo development potential.

Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development

This study investigates mRNA translation dynamics during mouse pre-implantation development, revealing novel mechanisms of translational selectivity and the role of Eif1ad3 in ribosome biogenesis.

Single-nucleotide Resolution Epitranscriptomic Profiling Uncovers Dynamic m6A Regulation in Bovine Preimplantation Development

This study uses SAC-seq to map m6A modifications at single-nucleotide resolution in bovine preimplantation embryos, identifying a critical m6A site in RPL12 that regulates translation and development.

METTL7A improves bovine IVF embryo competence by attenuating oxidative stress

This paper identifies METTL7A as a gene that enhances bovine IVF embryo development by reducing oxidative stress and mitochondrial damage.

Establishment of bovine extraembryonic endoderm cells

This study establishes a method to derive and culture bovine extraembryonic endoderm cells (bXENs) using a chemical cocktail, revealing their role in maintaining stemness and supporting embryo development.

DNA replication in early mammalian embryos is patterned, predisposing lamina-associated regions to fragility

The paper shows that DNA replication patterns in early mammalian embryos are established early, with late replicating regions near the nuclear lamina showing fragility and predisposing long genes to genetic changes.

Single-cell transcriptional landscapes of bovine peri-implantation development

The paper maps the single-cell transcriptomes of bovine peri-implantation development to identify cell lineages and molecular mechanisms involved in early pregnancy success and failure.

In Vitro Culture Alters Cell Lineage Composition and Cellular Metabolism of Bovine Blastocyst

The paper uses single-cell transcriptomics to compare bovine blastocysts cultured in vivo versus in vitro under different conditions, revealing how culture affects cell lineage and metabolism.

Bovine blastocyst like structures derived from stem cell cultures

The paper develops a method to generate bovine blastocyst-like structures (blastoids) from stem cells, which mimic blastocysts in morphology and transcriptomes.

Establishment of Bovine Trophoblast Stem Cells

This study uses a chemical cocktail to derive and culture bovine trophoblast stem cells, which retain developmental potency.

High-resolution Ribosome Profiling Reveals Translational Selectivity for Transcripts in Bovine Preimplantation Embryo Development

This study uses low-input ribosome profiling to analyze translational selectivity in bovine oocytes and preimplantation embryos, revealing four modes of translational regulation and their temporal dynamics.

The H3.3 chaperone Hira complex orchestrates oocyte developmental competence

This study shows that the Hira complex is essential for oocyte developmental competence by maintaining transcriptional silencing through H3.3 deposition and chromatin modifications.

The landscape of accessible chromatin in quiescent and post-myocardial infarction cardiac fibroblasts

This study examines chromatin accessibility and gene expression in cardiac fibroblasts before and after myocardial infarction using ATAC-seq and RNA-seq, revealing correlations between promoter accessibility and gene expression, and identifying potential transcription factors involved in differential gene expression.