bioRxivNeurosciencePreprint
3D reconstruction of cell nuclei in a full Drosophila brain
Shang MuiD, Szi-chieh YuiD, Nicholas L. TurneriD, Claire E. McKellariD, Sven DorkenwaldiD, Forrest CollmaniD, Selden KoolmaniD, Merlin Moore, Sarah Morejohn, Ben Silverman, Kyle Patrick Willie, Ryan Willie, Doug Bland, Austin BurkeiD, Zoe C. AshwoodiD, Kyle Luther, Manuel CastroiD, Oluwaseun Ogedengbe, William SilversmithiD, Jingpeng WuiD, Akhilesh HalageriiD, Thomas MacrinaiD, Nico KemnitziD, Mala MurthyiD, H. Sebastian SeungiD
- Citations
- 20
Abstract
We reconstructed all cell nuclei in a 3D image of a Drosophila brain acquired by serial section electron microscopy (EM). The total number of nuclei is approximately 133,000, at least 87% of which belong to neurons. Neuronal nuclei vary from several hundred down to roughly 5 cubic micrometers. Glial nuclei can be even smaller. The optic lobes contain more than two times the number of cells than the central brain. Our nuclear reconstruction serves as a spatial map and index to the cells in a Drosophila brain.
- organism: Drosophila
- assay: imaging
- research focus: spatial domain detection
- approach: experimental assay
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Automated Reconstruction of a Serial-Section EM Drosophila Brain with Flood-Filling Networks and Local Realignment
Peter H. Li, Larry F. Lindsey, … Viren Jain·bioRxiv·Oct 7, 2020
- Fast Assembling of Neuron Fragments in Serial 3D Sections
Hanbo Chen, Daniel Maxim Iascone, … Hanchuan Peng·Brain InformaticsPeer-reviewed·Apr 1, 2017
- Dense reconstruction of brain-wide neuronal population close to the ground truth
Zhou Hang, Li Shiwei, … Zeng Shaoqun·bioRxiv·Jan 2, 2018
- Connectome-driven neural inventory of a complete visual system
Aljoscha Nern, Frank Loesche, … Michael B. Reiser·NaturePeer-reviewed·Mar 26, 2025
- A Connectome of the Adult Drosophila Central Brain
C. Shan Xu, Michał Januszewski, … Ian A. Meinertzhagen·bioRxiv·Jan 21, 2020
- A segmentation scheme for complex neuronal arbors and application to vibration sensitive neurons in the honeybee brain
Hidetoshi Ikeno, Ajayrama Kumaraswamy, … Hiroyuki Ai·Frontiers in NeuroinformaticsPeer-reviewed·Sep 26, 2018