William Silversmith

Connectomic reconstruction from hippocampal CA3 reveals spatially graded mossy fiber inputs and selective feedforward inhibition to pyramidal cells

The paper maps large-scale neuronal connections in mouse hippocampal CA3, revealing spatially graded mossy fiber inputs and a feedforward inhibitory circuit targeting specific pyramidal cells.

CAVE: Connectome Annotation Versioning Engine

CAVE enables reproducible connectome analysis in large-scale datasets during ongoing annotation and proofreading.

NEURD offers automated proofreading and feature extraction for connectomics

NEURD automates proofreading and feature extraction from connectomics data to improve accuracy and accessibility of neural morphology analyses.

Functional connectomics spanning multiple areas of mouse visual cortex

The paper presents a functional connectomics dataset of mouse visual cortex, combining calcium imaging with EM reconstruction to study neural circuits and their connectivity.

Functional connectomics reveals general wiring rule in mouse visual cortex

Functionally similar neurons in mouse visual cortex connect preferentially across layers and areas, including feedback, and feature tuning, not receptive-field position, predicts synapses beyond axon-dendrite proximity.

An unsupervised map of excitatory neurons' dendritic morphology in the mouse visual cortex

The paper uses graph-based machine learning to analyze dendritic morphology of excitatory neurons in mouse visual cortex, finding a continuous morphological landscape rather than discrete types.

Predicting modular functions and neural coding of behavior from a synaptic wiring diagram

The paper uses a larval zebrafish connectome to predict neural coding of behavior and shows statistical matches with calcium imaging data.

Neuronal wiring diagram of an adult brain

The paper presents the first neuronal wiring diagram of a whole adult Drosophila brain, revealing synaptic connections and circuit mechanisms underlying sensorimotor behaviors.

Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy

This paper improves TEM imaging speed by using beam deflection to capture multiple image tiles, increasing efficiency for millimeter-scale brain tissue imaging.

A scalable and modular computational pipeline for axonal connectomics: automated tracing and assembly of axons across serial sections

The paper introduces a computational pipeline for automated tracing and assembly of axons across serial sections, validated on centimeter-scale data for orientation analysis and proofreading.

The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice

The paper investigates the synaptic connectivity of thick tufted layer 5 pyramidal neurons in the mouse visual cortex, revealing their local inhibitory and long-range excitatory connections.

Integrating EM and Patch-seq data: Synaptic connectivity and target specificity of predicted Sst transcriptomic types

The paper predicts MET-type identities from EM data and shows they have distinct connectivity patterns and synapse targets.

Cell-type-specific inhibitory circuitry from a connectomic census of mouse visual cortex

Electron microscopy of 1352 neurons in a mouse visual cortex column reveals widespread cell-type-specific inhibition, a basket-cell-targeting disinhibitory class, and interneuron motif groups sharing excitatory targets.

Binary and analog variation of synapses between cortical pyramidal neurons

The paper maps synaptic connectivity between cortical pyramidal neurons and shows synapse size is modeled by a combination of binary and analog variables.

Special Nuclear Layer Contacts Among Starburst Amacrine Cells in the Mouse Retina

The paper identifies novel nuclear layer contacts between starburst amacrine cells in the mouse retina through detailed morphological analysis of electron microscopic data.

Perisomatic Features Enable Efficient and Dataset Wide Cell-Type Classifications Across Large-Scale Electron Microscopy Volumes

The paper uses electron microscopy data to classify cell types in the neocortex based on perisomatic features, showing that these features are sufficient for accurate classification even in large datasets.

FlyWire: Online community for whole-brain connectomics

FlyWire enables collaborative proofreading of whole-brain connectomes through an online platform, demonstrating its utility in reconstructing and analyzing mechanosensory neuron circuits.

3D reconstruction of cell nuclei in a full Drosophila brain

The paper reconstructs 3D cell nuclei in a Drosophila brain, identifying over 133,000 nuclei, mostly neurons, and highlights differences in nuclear size and cell density across brain regions.

Petascale neural circuit reconstruction: automated methods

Cloud-run convolutional nets aligned 19,939 serial EM sections of mouse visual cortex despite cracks and folds, segmented neurons and detected 523 million synapses; code and the reconstruction are public.

Oligodendrocyte precursor cells prune axons in the mouse neocortex

The paper identifies oligodendrocyte precursor cells (OPCs) as major contributors to axon pruning in the mouse neocortex through phagocytic activity.

The Neural Basis for a Persistent Internal State in Drosophila Females

This study identifies pC1-Alpha neurons in Drosophila females that drive persistent behaviors through recurrent neural connectivity.

Multiscale and multimodal reconstruction of cortical structure and function

The paper presents a semi-automated reconstruction of mouse primary visual cortex from electron microscopy data, revealing structural and functional relationships in cortical circuits.

Chandelier cell anatomy and function reveal a variably distributed but common signal

The paper maps chandelier cell synapses onto pyramidal neurons and finds variability in synaptic input correlated with target neuron features, while showing functional correlation with arousal state.

Digital museum of retinal ganglion cells with dense anatomy and physiology

The paper presents a digital resource of mouse retinal ganglion cell dendritic arbors and visual responses, identifies sublaminae, clusters cells, and finds sustained responses in one sublamina.