Establishment of Bovine Trophoblast Stem Cells

Yinjuan Wang; Hao Ming; Leqian Yu; Jie Li; Linkai Zhu; Hai‐Xi Sun; Carlos A. Pinzón-Arteaga; Jun Wu; Zongliang Jiang

doi:10.1016/j.celrep.2023.112439

Developmental BiologyCell ReportsMay 1, 2023

Peer-reviewed journal article

Establishment of Bovine Trophoblast Stem Cells

Yinjuan Wang iD, Hao Ming iD, Leqian Yu iD, Jie Li iD, Linkai Zhu iD, Hai‐Xi Sun iD, Carlos A. Pinzón-Arteaga iD, Jun Wu iD, Zongliang Jiang iD

Abstract

Here we report that a chemical cocktail (LCDM: hLIF, CHIR99021, DiM and MiH) previously used for extended potential pluripotent stem cells enables the de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs exhibit transcriptomic and epigenetic features characteristic of trophectoderm cells from bovine embryos and retain developmental potency to differentiate into mature trophoblast cells.

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