Microglia inhibition rescues developmental hypofrontality in a mouse model of mental illness

Cognitive deficits, core features of mental illness, largely result from dysfunction of prefrontal-hippocampal networks. This dysfunction emerges already during early development, before a detectable behavioral readout, yet the cellular elements controlling the abnormal maturation are still unknown. Combining in vivo electrophysiology and optogenetics with neuroanatomy and pharmacology in neonatal mice mimicking the dual genetic - environmental etiology of psychiatric disorders, we identified pyramidal neurons in layer II/III of the prefrontal cortex as key elements causing disorganized oscillatory entrainment of local circuits in beta-gamma frequencies. Their abnormal firing rate and timing result from sparser dendritic arborization and lower spine density. Pharmacological modulation of aberrantly hyper-mature microglia rescues morphological, synaptic and functional neuronal deficits and restores the early circuit function. Elucidation of the cellular substrate of developmental miswiring related to later cognitive deficits opens new perspectives for identification of neurobiological targets, amenable to therapies.\n\nHighlightsO_LIMice mimicking the etiology of mental illness have dysregulated prefrontal network\nC_LIO_LIStructural and synaptic deficits cause abnormal rate and timing of pyramidal firing\nC_LIO_LIWeaker activation of prefrontal circuits results from deficits of pyramidal neurons\nC_LIO_LIRescue of microglial function restores developing prefrontal circuits\nC_LI