Wastewater Treatment and Nitrogen Removal

Beyond Conveyance: Assessing the Contribution of Process-Critical Bacteria from Urban Sewer Systems to Wastewater Treatment Plants

The paper quantifies biomass loads from sewer systems to wastewater treatment plants using microbial community data and mass balances, showing gravity sewers produce higher biomass loads than pressure systems.

Genomic and physiological characterization of Neobacillus ammoniipjilus sp. nov. reveals versatile inorganic nitrogen utilization and ammonia-mitigation potential

This paper characterizes a novel bacterial strain, Neobacillus ammoniiphilus, highlighting its ability to utilize various inorganic nitrogen sources and mitigate ammonia.

From dynamic transcripts to stable proteins: multi-omics revealed regulatory buffering of Candidatus Accumulibacter under cyclic conditions

This study uses multi-omics data to show that Candidatus Accumulibacter buffers transcriptional dynamics at the protein level, maintaining stable proteomes during cyclic EBPR operations.

Complete ammonia-oxidizing Nitrospira and ammonia-oxidizing archaea as biological supplements for freshwater aquaria

This study evaluates the effectiveness of nitrifying supplements containing Nitrospira and ammonia-oxidizing archaea in freshwater aquaria, showing they reduce ammonia and nitrite more effectively than conventional supplements.

A Saccharimonadia epibiont interacts with the polyphosphate-accumulating bacterium Candidatus Phosphoribacter in wastewater treatment plants

The paper identifies a novel Saccharimonadia genus associated with a polyphosphate-accumulating bacterium in wastewater treatment plants and explores their ecological and metabolic interactions.

Dark CO2 Fixation via the Ethylmalonyl-CoA Pathway Establishes Metabolic Parity: A Stoichiometric Basis for Compounding Ecosystem Shifts

The paper shows glycogen-accumulating organisms use the ethylmalonyl-CoA pathway for CO2 fixation to achieve metabolic parity with polyphosphate-accumulating organisms, leading to community shifts through stoichiometric advantages.

Genome-resolved and kinetic evidence for low-DO comammox-anammox synergy and acetate-stimulated nitrate reduction in IFAS biofilms

This study shows that low dissolved oxygen and acetate amendment enhance anammox activity in IFAS biofilms by supporting microbial synergy and nitrate reduction.

Fish load impacts biofilter microbial communities and nitrifier populations during establishment of freshwater home aquaria

This study shows that varying fish loads in freshwater aquaria affect microbial community composition and nitrifier populations, with higher loads promoting distinct microbial communities and increased ammonia and nitrite levels.

A new player in the biochemistry of Anammox bacteria: a multidomain HAO-like protein

This paper identifies a novel HAO-like protein in anammox bacteria that may convert hydroxylamine to hydrazine, potentially playing a role in the anammox cycle.

Horizontal gene transfer drives DNRA capacity in wastewater treatment systems

The paper examines genomic architecture in anammox bacteria, finding that only Candidatus Brocadia sinica has a compact genomic island for iron genes, while dispersed species show expression uncoupling between hzs and iron genes.

A unique compact genomic island co-localizing iron and anammox genes in Candidatus Brocadia sinica, but not in other species

Evidence that the Kuenenia stuttgartiensis encapsulin does not protect against NO damage

The paper tests whether the Kuenenia stuttgartiensis encapsulin protects against NO damage and finds no evidence of such protection.

Ecophysiological niche expansion driven by biofilm lifestyle in an archaeal soil nitrifier

This study shows that biofilm growth enhances the ecological resilience and reduces nitrous oxide emissions in ammonia-oxidizing archaea under various environmental conditions.

The molecular mechanism and activity of Kuenenia stuttgartiensis hydrazine synthase

This study experimentally investigates the molecular mechanism of hydrazine synthase in Kuenenia stuttgartiensis, confirming the second half-reaction of hydrazine synthesis from ammonium and hydroxylamine.

Quantification of hydrazine in biochemical assays and anammox bacteria using LC-MS

This paper develops and validates an LC-MS method to quantify hydrazine produced by anammox bacteria and isolated hydrazine synthase with high accuracy and precision.

Differential Maillard Sensitivity Of Exoproteins Favors Keratin Recovery During Sludge Biopolymer Extraction

The paper assesses protein recovery from alkali-treated EPS in activated sludge, finding keratin dominates due to its resistance to Maillard reactions, while bacterial proteins are modified and less recoverable.

Autoinducer-2 and acyl homoserine lactones have contrasting effects on ammonia and nitrite-oxidizing sludge

This study shows that AHLs boost ammonia oxidation while AI-2 suppresses it but enhances nitrite oxidation, suggesting they regulate nitrification differently.

Two Small Proteins Enable N2O Reduction in Acidic Environments

The paper identifies two proteins, NosP and NosQ, that enable N2O reduction in acidic environments by protecting the NosZ enzyme from low pH.

Flavobacteria buffer nitrous oxide emissions from partial denitrifiers in coastal sediments

The paper identifies Flavobacteria in coastal sediments that reduce nitrous oxide emissions by having low substrate affinity, contradicting prior assumptions and suggesting they buffer nitrogen cycling.

Metagenomes and Metagenome-Assembled Genomes from Microbial Communities in a Biological Nutrient Removal Plant Operated at Los Angeles County Sanitation District (LACSD) with High and Low Dissolved Oxygen Conditions

The paper analyzes metagenomes and MAGs from a wastewater treatment plant under varying DO conditions to study microbial adaptation to energy-efficient processes.

Machine-learning informs mitigation strategies for nitrous oxide emissions from wastewater operations

This paper uses machine learning to predict and mitigate nitrous oxide emissions from wastewater treatment systems by analyzing process variables and their impact on emissions.

Measuring Temporal Variations of Nucleotide Pools in Microbial Granular Biofilm Performing Enhanced Biological Phosphorous Removal

The paper develops a metabolomics workflow to measure temporal variations in nucleotide pools in EBPR biofilms, revealing dynamic changes in energy charge during metabolic processes.

Non-canonical reductive nitrous oxide production pathways in a seasonally stratified lake basin

The paper identifies non-canonical nitrous oxide production pathways in a lake through isotope analysis and incubation experiments, showing bacterial denitrification as dominant with contributions from chemo- and fungal denitrification.

Hypoosmolarity inhibits archaeal ammonia oxidation

This study shows hypoosmolarity inhibits archaeal ammonia oxidation while promoting bacterial ammonia oxidation in different environments.

A new nitrogen biochemical process: Oxidization of ammonium into nitrogen gas using nitrate

This study discovers a new anaerobic process where nitrate directly oxidizes ammonium to nitrogen gas, achieving high nitrogen removal rates.

Influence of carbon-to-nitrogen ratio on the denitrifying activities of a methanol-fed, marine recirculating denitrification reactor, and the evolution of the bacteria community during the operating process.

This study examines how varying carbon-to-nitrogen ratios affect denitrification in a marine reactor and microbial community evolution over 31 weeks.

Coupling Anaerobic Oxic and Integrated Fixed Activated Sludge Processes for Removal and Transformation of Refractory Organic Matter in Livestock and Poultry Digestate

This study compares anaerobic-oxic and IFAS systems for treating livestock digestate, finding IFAS achieves higher organic removal and promotes oxidative fragmentation of organic matter.

Microbial community characteristics and stable relationships centered on anammox bacteria revealed by global-scale analysis

This study identifies stable microbial relationships with anammox bacteria using global data, revealing key companion bacteria and their roles in anammox metabolism.

Multi-omics highlights challenges in assessing the composition and performance of microbial consortia for commercial applications

This study uses multi-omics to analyze a microbial consortium for wastewater treatment, revealing shifts in community composition and nitrogen cycling under different environmental conditions.

Membrane vesicles of Shewanella oneidensis MR-1 enhance denitrification growth in a species-selective manner

This study shows that membrane vesicles from Shewanella oneidensis MR-1 enhance denitrification in a species-selective way by promoting bacterial attachment and influencing metabolic activity.