Wastewater Treatment and Nitrogen Removal

Oxygen production as an electron overflow pathway in ammonia-oxidizing archaea

The paper shows that oxygen production in ammonia-oxidizing archaea is an electron overflow pathway under anoxic conditions, not a response to low oxygen.

Overexpression of a Gene That Modulates Cyclic-di-GMP Enhances Granulation in Mycobacterium smegmatis

This study shows that overexpressing dcpA in Mycobacterium smegmatis enhances granulation and granule stability, suggesting c-di-GMP modulation plays a key role in microbial aggregation.

Dual-loop involving microbial single-cell protein production from soybean-processing wastewater and effluent-based refinement for circular bioeconomy applications

This paper presents a dual-loop bioprocess converting soybean-processing wastewater into microbial single-cell protein with nutrient recovery and product refinement.

Metagenomes and Metagenome-Assembled Genomes from Microbial Communities in a Biological Nutrient Removal Plant Operated at Hamptons Road Sanitation District (HRSD) with High and Low Dissolved Oxygen Conditions

The paper analyzes metagenomes and metagenome-assembled genomes from microbial communities in a wastewater treatment plant under varying dissolved oxygen conditions to understand microbial adaptation to low-DO environments.

Distinct denitrification phenotypes in closely related bacteria: clues to understanding variations in nitrite accumulation among Stutzerimonas strains

The paper identifies three nitrite accumulation phenotypes in Stutzerimonas strains and links them to differences in nitrate reduction and nitrite reductase genes.

Contrasting effects of glutamate and branched-chain amino acid metabolism on acid tolerance in a Castellaniella isolate from acidic groundwater

This study identifies glutamate and branched-chain amino acid metabolism as key factors in acid tolerance of a denitrifying bacterium from acidic groundwater.

Unraveling Candidatus Dermatophostum as a Novel Genus of Polyphosphate-Accumulating Organisms for High-Strength Wastewater Treatment

This study identifies a new PAO genus, Candidatus Dermatophostum, in wastewater treatment systems and proposes a ppk1-based classification framework for Dermatophilaceae PAOs.

Kinetic Plasticity of Nitrite-Oxidizing Bacteria Containing Cytoplasmic Nitrite Oxidoreductase

This study shows that Nitrobacter can adjust its nitrite affinity based on nitrite concentration and pH, demonstrating adaptive plasticity in cNXR NOB.

Discovering Polyphosphate and Polyhydroxyalkanoate-Accumulating Organisms Across Ecosystems: Phenotype-targeted Genotyping via FACS-sequencing

This study introduces TriFlow-Seq, a method combining FACS and 16S rRNA sequencing to identify PHA-PAOs in EBPR systems and soil, revealing new candidates and their ecological roles.

Denitrification is a community trait with partial pathways dominating across microbial genomes and biomes

The paper analyzes denitrification gene distribution across microbial genomes and biomes, finding partial denitrifiers dominate and complete denitrifiers are more metabolically flexible.

Building Predictive Understanding of Microbial Ecology by Bridging Microbial Growth Kinetics and Microbial Population Dynamics

The paper develops a modeling approach integrating microbial growth kinetics and population dynamics to predict microbial community behavior in activated sludge systems.

Hexavalent Chromium Inhibits Nitrate-Dependent Anaerobic Methane Oxidation While Enriching Denitrifiers: Insights into Microbial Interactions for Simultaneous Methane, Nitrate and Chromate Removal

This study investigates how hexavalent chromium affects nitrate-dependent anaerobic methane oxidation, finding it inhibits methane oxidation and reduces key microbial populations.

Nitric oxide-forming nitrite reductases in the anaerobic ammonium oxidizer Kuenenia stuttgartiensis

The paper identifies two active nitrite reductases in K. stuttgartiensis that produce nitric oxide, contributing to the anammox reaction.

Nitrous oxide production, mechanisms, and modeling from a denitrifying phosphorus removal bioreactor

This study investigates N2O production in a wastewater treatment reactor, identifies key microbial pathways, and models N2O generation using metagenomic data.

Linking Oxygen-Induced Oxidative Stress to Resource Recovery by Enhancing the Production of Extracellular Polymeric Substances in Activated Sludge Microbial Communities

This study enhances EPS production in activated sludge by manipulating oxygen exposure to increase oxidative stress, resulting in a 90.5% higher EPS yield.

Sediments from a seasonally euxinic coastal ecosystem show high nitrogen cycling potential

The paper examines nitrogen cycling in sediments of a seasonally euxinic coastal ecosystem, finding high nitrogen removal potential through denitrification and DNRA.

Establishing a co-culture aggregate of N-cycle bacteria to elucidate flocculation in biological wastewater treatment

The paper demonstrates the feasibility of using a simplified N-cycle bacterial consortium to study bioflocculation in wastewater treatment by forming and observing microcolonies and macroclusters under controlled conditions.

Mitigating nitrous oxide emission by an ultra-fast bioprocess enabling the removal of high concentration N2O

This paper develops a novel bioprocess using a down-flow hanging sponge reactor to efficiently remove high concentrations of nitrous oxide, achieving over 96% removal efficiency for 300 ppm N2O.

Multiple extracellular polymeric substances pathways expressed by Accumulibacter and the flanking community during aerobic granule formation and after influent modification

The paper studies microbial community changes and extracellular polymeric substances during aerobic granule formation and under phosphate concentration modification.

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

This study investigates anammox bacteria adaptation to high temperatures, finding increased ladderane lipid cyclization and proteomic changes as key mechanisms.

Resource Recovery from Wastewater By Directing Microbial Metabolism Toward Production of Value-added Biochemicals

The paper tests if fluctuating oxygen levels in wastewater treatment can boost production of valuable biochemicals by altering microbial metabolism.

Emergence and disruption of cooperativity in a denitrifying microbial community

This study identifies pathway partitioning as a mechanism for complete denitrification in microbial communities and shows how imbalances in substrate utilization can disrupt cooperativity and lead to nitrous oxide emissions.

Occurrence and temporal dynamics of denitrifying protist endosymbionts in the wastewater microbiome

The paper investigates denitrifying protist endosymbionts in wastewater treatment plants, finding their presence in 20-50% of facilities and highlighting temporal fluctuations and incomplete denitrification pathways.

Lotka-Volterra Dynamics Facilitate Sustainable Biocontrol of Wastewater Sludge Bulking

The paper uses Lotka-Volterra dynamics to model the biocontrol of wastewater sludge bulking by protists against filamentous bacteria.

Selective enrichment of high-affinity clade II N2O-reducers in a mixed culture

The paper shows clade II N2O-reducers outcompete clade I under low N2O availability, and coexist under high N2O, with cobalamin interactions influencing community composition.

Meta-omic insights into active bacteria mediating N2O mitigation and dissimilatory nitrate reduction to ammonium in an ammonia recovery bioreactor

The paper uses metagenomics and metatranscriptomics to identify bacteria involved in N2O mitigation and ammonification in a bioreactor, showing how oxygen levels affect their activity.

From gene to structure: Unraveling genomic dark matter in Ca. Accumulibacter

The paper develops a method to annotate genomic dark matter in Ca. Accumulibacter by integrating pangenome analysis, protein structure prediction, and metatranscriptomics, significantly improving gene annotation coverage.

Comparative metagenomic analysis provides novel insights into the ecophysiology of Myxococcota in activated sludge systems

This study uses metagenomic analysis of activated sludge samples to investigate the ecophysiological traits of Myxococcota, revealing their predatory capabilities and metabolic versatility.

Denitrification genotypes of endospore-forming Bacillota

The paper identifies denitrification genotypes in Bacillota using HMMs and genome-resolved metagenomics, finding complete denitrification potential in several MAGs and 17 genera.

Comparative metaproteomics demonstrates different views on the complex granular sludge microbiome

The paper compares metaproteomic and genomic approaches to study the aerobic granular sludge microbiome, highlighting differences in insights and the importance of reference databases.