RNA Interference and Gene Delivery

The Aquarius/EMB-4 helicase licenses co-transcriptional gene silencing

This study identifies the Aquarius/EMB-4 helicase as essential for initiating small RNA-induced co-transcriptional gene silencing in C. elegans by facilitating HRDE-1 complex engagement with nascent transcripts.

Characterization of SID-1-dependent and independent intergenerational RNA transport pathways in Caenorhabditis elegans

The paper identifies three independent pathways for dsRNA transport from germline to embryos in C. elegans, involving Sid genes and RME-2, and discusses their roles in RNAi and potential transgenerational inheritance.

Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes

The paper develops a compact shRNA expression system using retroviral miRNA gene architecture, reducing coding space needed for shRNA expression by over two-fold compared to U6/H1 promoters.

shRNA mediated inhibition of Cdc42 gene expression in Calu-6 lung cancer cells

This study inhibits Cdc42 gene expression in Calu-6 lung cancer cells using lentiviral shRNA and shows reduced cell viability and gene expression.

SID-1 domains important for dsRNA import in C. elegans

The paper identifies conserved microdomains in C. elegans SID-1 critical for dsRNA import and shows SID-1 functions in intestinal environmental RNAi.

Each cell needs long double-stranded RNA for silencing by feeding RNAi in C. elegans

The paper shows that long dsRNA binding by RDE-4 is required for systemic RNAi in C. elegans, contradicting previous findings using repetitive transgenes.