Poxvirus research and outbreaks

This study uses CAGE and long-read RNA sequencing to analyze the transcriptional architecture of human monkeypox virus, identifying alternative TSSs, TESs, promoter elements, and novel genes.

The paper identifies a conserved heparan sulfate binding domain in MPXV H3 using AI and MD simulations, validates its role in viral adhesion, and demonstrates a protein inhibitor that disrupts this interaction, inhibiting infection in vitro and in vivo.

The paper presents the 3.8 Å cryo-EM structure of the VACV DNA polymerase holoenzyme and shows its structural similarity to MPXV, with transient E9-D4 interactions and conformational flexibility.

The paper characterizes the monkeypox outbreak in southern Spain using whole genome sequencing and phylogenetic analysis, identifying mutations potentially linked to drug resistance.

The paper uses cryo-EM and AlphaFold to determine the structure of the poxvirus core, identifying A10 trimers as the key component of the palisade layer.

The paper identifies the palisade layer of the vaccinia virus core as composed of flexible A10 trimers using cryoET, STA, and AlphaFold2.

The paper analyzes Monkeypox virus genomes from the 2022 outbreak and compares them to historical and related sequences to determine their origin and genetic characteristics.

This study analyzes 604 human Monkeypox virus genomes to examine genomic epidemiology and molecular evolution, identifying synteny breaks, selection pressures, and phylogenetic assignments to the West African clade.

The paper analyzes MPXV genomes and host-virus interactions to identify mutational hotspots, functional patterns, and potential drug repurposing targets for monkeypox treatment.

The paper reports genome sequences of Lumpy Skin Disease Virus from an outbreak in India, finding a distinct lineage with significant genetic variations compared to the reference genome.

The paper analyzes APOBEC3-driven mutagenesis in monkeypox virus genomes, finding over-representation of TC hotspots and under-representation of GC hotspots, suggesting potential for future human APOBEC3-mediated evolution.

The paper identifies a distinct phylogenetic cluster of monkeypox virus genomes, lineage A.2, which differs from the dominant lineage B.1 associated with the 2022 European outbreak.

The paper identifies two lineages of Monkeypox virus in the U.S. in 2021-2022, finding a common ancestor and evidence of APOBEC3 editing in West African strains.

The paper analyzes the innate immune response at injection sites after vaccination with a recombinant vaccinia vector, identifying immune signatures and gene expression patterns.

This paper uses next-generation sequencing to analyze cowpox virus outbreaks in humans and cats, finding high clonality in a human outbreak and partial transmission in a feline outbreak.