Molecular Biology Techniques and Applications

Click-qPCR provides an interactive, web-based tool for analyzing qPCR data without programming skills, enabling efficient processing and visualization of results.

qDATA provides an R-based tool for analyzing qRT-PCR data, offering statistical analysis and visualization of Ct values with a focus on improving reliability through biological replicates and technical replicates.

The paper evaluates how pre-sequencing RNA quality metrics (PMI and RIN) correlate with post-sequencing RNA quality metrics and gene expression in human brain tissue.

This paper demonstrates that a mixture of multiple blocker sequences can suppress PCR errors even with partial contaminating sequence information, improving the fidelity of PCR and related techniques.

The paper evaluates reference genes for qPCR normalization in a rat tendon injury model, identifying stable genes and highlighting unstable ones.

The paper compares RNA-ISH quantification methods in ovarian cancer samples, finding good concordance between automated tools and RNAscope, with RT-ddPCR showing less agreement.

The paper introduces a RT-qPCR assay to assess mRNA integrity in brain and subcellular samples, showing it outperforms RIN in correlation with housekeeping gene expression.

This study evaluates RNA integrity in horse hair follicles stored in RNAlater at different temperatures and finds no significant differences in RNA quality or quantity across conditions.

The paper develops a non-invasive DNA collection protocol for rat genotyping using rectal swabs, showing it produces reliable PCR results comparable to traditional methods.

The paper describes a modified dual preparatory method to improve RNA yield from laser microdissected human cancer tissue samples.

The paper characterizes a novel thermostable and processive reverse transcriptase from Anoxybacillus flavithermus, showing its potential for applications requiring heat-resistant RTs.

The paper introduces a mathematical framework for improving qPCR gene expression normalization by optimizing weighted aggregation of reference genes using scale-invariant functions.

The paper introduces a low-cost system for high-throughput RNA isolation from tissues, combining CryoGrid for storage/sampling with PIXUL-based methods using a PK buffer to enable efficient transcript analysis.

This paper introduces a novel hydrogel capsule-based digital PCR method that improves stability, sensitivity, and cost-effectiveness compared to traditional ddPCR.

The paper identifies stable reference genes for qRT-PCR in insect endosymbiotic models using transcriptomic data from Sitophilus oryzae.

The paper introduces an in-house RNA isolation method using magnetic silica beads that produces high-quality RNA comparable to commercial kits, without DNA digestion or separate small RNA kits.

The paper describes a plasmid, pHAPE, that allows the generation of DNA size markers through restriction enzyme digestions, reducing costs associated with commercial DNA ladders.

This paper develops an optimized RNA extraction method for rat intrinsic laryngeal muscles, achieving high-quality RNA suitable for RNA-seq.

The paper introduces and compares two Direct PCR methods for Arabidopsis and rice, finding Line-PCR to be faster, cheaper, and more accurate than existing methods.

The paper describes an optimized LCM protocol for extracting high-quality RNA from specific mouse brain regions.

This paper develops a method to estimate time since deposition of bloodstains using RNA quality metrics from degrading blood samples without amplification.

This study shows that delayed processing of blood samples affects mRNA-based biomarkers by altering transcript abundance in peripheral white blood cells.

This study shows that using a robust statistical method to select reference genes from randomly chosen candidates is as effective as selecting stable genes from RNA-Seq data for qPCR normalization.

This paper introduces VPdPCR, a method that enhances digital PCR's ability to detect multiple targets per sample, improving accuracy and reducing variance in chromosomal counting.

The paper develops a statistical model to estimate population allele frequency from qPCR ΔΔCq measures of bulk samples, showing improved precision over simple averaging.

The paper describes a cost-effective method for preparing cDNA synthesis and real-time PCR mastermixes that match commercial kits' efficiency, addressing financial barriers in low-resource settings.

This study evaluates miRNA reference genes in zebrafish across strains, developmental stages, and kidney disease models, identifying dre-miR-92b-3p as the most stable reference gene.

Auto-qPCR automates qPCR data analysis, reducing errors and time by providing a web-based tool for DNA quantification and gene expression analysis.

This paper identifies optimal reference genes for qPCR normalization in mesangial cells and podocytes under high-glucose and losartan treatment conditions.