Identification and Quantification in Food

Advancing global DNA-referencing of bushmeat in African tropical forests: a tool for identifying trade hotspots and dynamics in western and central Africa

The paper develops a DNA-typing reference database to trace bushmeat trade in Africa, identifying species and trade hotspots with high accuracy.

Hidden Biodiversity in Wildlife Trade Networks: DNA Barcoding Reveals Fish and Crocodilian Species in Commercialized Swim Bladders

This study uses DNA barcoding to identify hidden biodiversity in commercialized fish swim bladders, revealing four species including two Amazonian crocodilians.

Analytical Validation of Automated DNA Isolation from Meat Matrices for High-Quality PCR-Based Food Authentication

This paper validates an automated DNA extraction method for meat matrices, showing it produces high-quality DNA suitable for PCR-based food authentication.

High-Resolution Melting Analysis of Chloroplast Markers for Species Authentication and Fraud Detection in Commercial Acai and Jucara Products

This paper evaluates HRM analysis of chloroplast markers for species authentication of acai and jucara products, finding it more reliable than DNA sequencing in detecting mislabeling.

SPrOUT: A computational and targeted sequencing approach for mixed plant DNA identification with Angiosperms353

This paper introduces a computational and targeted sequencing approach for identifying plant species in mixed samples using the Angiosperms353 kit, achieving high accuracy and precision in both in-silico and mock mixtures.

A meta-review of DNA-based identification methods and mislabeling analysis of Eastern South Pacific seafood

This study reviews DNA-based seafood identification methods and finds a 24.8% mislabeling rate in Eastern South Pacific seafood, with many cases involving threatened shark species.

Improved Metagenomic Analysis for All-Food-Sequencing with AFS-MetaCache2: Illumina vs. Nanopore

This paper improves metagenomic analysis for food sequencing using AFS-MetaCache2, showing long-read sequencing and the new tool outperform existing methods in accuracy and efficiency.

Rapid on-site universal vertebrate species identification via multi-barcode nanopore sequencing

VeRIF-ID provides a rapid, on-site method for vertebrate species identification using nanopore sequencing of multiple DNA barcodes, achieving species-level accuracy in 91% of tested species.

Development of a sandwich ELISA for the detection of bovine A1 beta-casein

This paper develops a sandwich ELISA to detect A1 beta-casein in A2 milk, showing it can identify contamination at 1% A1 spike in a herd of A2A2 cows.

Tracing the illicit Peruvian delicacy: proof-of-concept multiplex-PCR for the simultaneous detection of seven commercial Macrobrachium prawn species

The paper develops a multiplex PCR assay to identify seven Macrobrachium prawn species and detects illegal trade in Peruvian prawns.

Sale of critically endangered sharks in the United States

This study analyzed shark meat products in the U.S. and found most were mislabeled, with some from critically endangered species and high mercury levels.

Development and validation of versatile species-specific primer assays for eDNA monitoring and authentication of 10 commercially important Peruvian marine species

The paper develops and validates species-specific primers for eDNA monitoring and authentication of 10 Peruvian marine species, achieving 100% accuracy and no cross-species amplification.

An updated DNA barcoding tool for Aloe vera and related CITES-regulated species

The paper introduces an updated DNA barcoding tool for Aloe vera and related CITES-regulated species, validated for accurate species identification using 189 nuclear markers.

Establishing a DNA reference library for the identification of elasmobranchs in the Indian Ocean using Oxford Nanopore Sequencing

This study develops a DNA reference library for elasmobranch species in Sri Lanka using Oxford Nanopore Sequencing for fisheries monitoring and conservation.

Fifty shades of white fish: DNA barcoding reveals widespread misidentification in sushi restaurants from Southern Brazil

This study uses DNA barcoding to reveal widespread misidentification of 'white fish' sushi in Southern Brazil, finding that one in five restaurants provided incorrect species information.

Molecular identification of whole squids and calamari at fairs and markets in regions of Latin America

The paper uses genetic sequencing of the rrnL gene to identify cephalopod species sold in Latin American markets and detect labeling errors.

Quantification of adulterated fox-derived components in meat Products by Drop Digital Polymerase Chain Reaction

This paper develops a ddPCR method to quantify fox-derived components in meat products and validates its accuracy through artificial adulteration testing.

A robust set of qPCR methods to evaluate adulteration in major spices and herbs

This paper develops qPCR methods to detect adulterants in six spices and herbs, validating their sensitivity and versatility for authenticity testing.

Statistical modelling of seafood fraud in the Canadian supply chain

The paper uses statistical models to analyze seafood fraud in Metro Vancouver, finding consistent trends with past studies and highlighting the need for better management.

Optimizing PCR using mtDNA Universal Primers for Detecting Indigenous Domestic Animal Species from Pakistan

The paper develops a PCR method using mtDNA universal primers to detect and differentiate indigenous domestic animal species in Pakistan, providing a tool for meat traceability and combating adulteration.

DNA barcoding revealed a high percentage of mislabeling in commercial fish products: the first empirical survey in South Texas

The paper uses DNA barcoding to analyze fish samples from South Texas markets, finding 20.6% mislabeling and 38.1% non-compliance with FDA market names.

Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

The paper develops a portable DNA barcoding method using deep learning to identify shark and ray species in trade, achieving 79.41% accuracy for 28 species.

Best BLAST hit alone cannot be used as evidence of fraud

The paper reanalyzes data from a study claiming fraud in fish labeling and finds that the best BLAST hit alone is insufficient evidence for fraud.

DNA metabarcoding using new rbcL and ITS2 metabarcodes collectively enhance detection efficiency of medicinal plants in single and polyherbal formulations

Shark-dust: High-throughput DNA sequencing of processing residues unveils widespread trade in threatened sharks and rays

Shark-dust DNA sequencing reveals widespread trade in threatened sharks and rays, identifying 54 taxa including many CITES-listed species.

Comprehensive analysis using DNA metabarcoding, PCR, and HPLC unveils the adulteration in Brahmi herbal products

This study compares DNA metabarcoding, PCR, and HPLC to detect Brahmi adulteration in herbal products, finding varying detection rates across methods.

Accurate determination of meat mass fractions using DNA measurements for quantifying meat adulteration

This study develops ddPCR assays to accurately quantify pork and beef DNA in meat products, achieving low detection limits and reliable quantification for adulteration detection.

Using target capture to address conservation challenges: population-level tracking of a globally-traded herbal medicine

This study uses target capture sequencing to identify and trace the geographic origin of Anacyclus pyrethrum samples in global trade, showing its effectiveness over traditional DNA barcoding methods.

Limitations of DNA barcoding in determining the origin of smuggled seahorses and pipefishes

The paper shows DNA barcoding cannot reliably determine the region of origin for smuggled seahorses and pipefishes due to insufficient genetic resolution.

Species substitution in the meat value chain by high-resolution melt analysis of mitochondrial PCR products

This study uses PCR-HRM analysis of mitochondrial genes to detect meat substitution in Nairobi, Kenya, finding 10.3% of samples were substituted with cheaper species.