Discover, rate, and verify biology preprints.

A mitochondrial orphan protein contributes to sperm development in bivalves

This study identifies a mitochondrial orphan protein, MORF, that contributes to sperm development in bivalves by regulating mitochondrial organization during spermatogenesis.

XRN1 couples RNA granule remodelling to global mRNA decay triggered by the activity of a viral endoribonuclease

The paper investigates how XRN1 links RNA granule remodeling to global mRNA decay triggered by the viral endoribonuclease vhs, revealing a sequential model of translational arrest and degradation.

The Molecular Interplay of Tau and Motor Proteins on Microtubules

This study investigates how tau protein regulates motor proteins on microtubules using single-molecule imaging, cryo-EM, and MD simulations, revealing distinct mechanisms for inhibiting kinesin-1 and dynein.

RubisCO-encoding methanotrophs elicit a coordinated transcriptional response to carbon dioxide limitation.

This paper shows that Methylococcus capsulatus uses CO2 for growth and activates a transcriptional response to CO2 limitation through the CbbR regulator.

Isolation of functional endogenous clathrin-coated vesicles and in vitro reconstitution of pure clathrin cages

The paper isolates functional endogenous clathrin-coated vesicles and reconstitutes pure clathrin cages from porcine brain, free of lipid contaminants.

Optimization of a Subcutaneous Epithelioid Hemangioendothelioma Xenograft Model for Evaluation of Preclinical Therapeutics

This study optimizes a subcutaneous xenograft model for EHE using patient-derived cells, showing that low cell densities produce reproducible tumors sensitive to mTOR inhibition.

Systematic comparison of genetically encoded F-actin probes for visualizing actomyosin dynamics in vivo using C. elegans

The paper compares three F-actin probes in C. elegans, finding mStayGold offers best brightness and photostability, while mNeonGreen is suitable for photobleaching applications.

Evaluating the Identifiability of Temporal Delay and Response Attenuation in Endometrial Decidualization

The paper evaluates the identifiability of temporal delay and response attenuation in endometrial decidualization using computational models and datasets, showing that distinguishing these mechanisms requires careful analysis.

Unified cell segmentation for spatial transcriptomics via transcript reassignment

Tracel improves cell segmentation in spatial transcriptomics by assigning transcripts to cells using optimal transport, enhancing cell-type separation and reducing contamination.

Histopathological and virus-associated features of Boid Inclusion Body Disease in Boa constrictor

This study examines histopathological and viral protein expression features of Boid Inclusion Body Disease in boa constrictors, finding higher IB and NP scores in recent cases and no significant link between NP scores and inflammation.

GPBench: A benchmark framework for genomic prediction

GPBench provides a standardized benchmark framework for genomic prediction, evaluating 21 models across 16 species and offering tools for analysis and comparison.

A microstructural analysis of water intake patterns associated with light phase food and water restriction in male and female rats.

The paper examines how food and water intake patterns in rats change under light phase restrictions, finding that food access timing more strongly influences water intake than water access timing influences food intake.

Stochastic gene expression drives state switching and phenotypic heterogeneity in RNA repair

The paper shows stochastic RNA repair in E. coli leads to phenotypic heterogeneity and state switching in antibiotic responses.

Phage-Inducible Chromosomal Jumbo Islands (PICJIs) spreading antimicrobial resistance, host-adaptation factors and defense systems, a novel satellite family of nucleus-forming Chimalliviridae

The paper identifies PICJIs as large mobile genetic elements in E. coli and Salmonella that spread antimicrobial resistance and defense systems via a specific bacteriophage.

MicrobeRT: A Framework and Suite of Fine-tuned Language Models for Genomic Sequences

MicrobeRT is a framework of fine-tuned language models for metagenomic analysis, achieving strong performance in taxonomic classification, antibiotic resistance detection, and pathogenicity assessment.

Aging activates latent enhancers that can be reversed withexercise

This study identifies age-acquired enhancers in mouse hearts and shows that exercise can reverse their activation.

Rapid Electro-optic Two-photon Bessel Light Sheet Fluorescence Lifetime Imaging for Tissue Volume Interrogation

This paper introduces a new two-photon fluorescence lifetime imaging technique for rapid volumetric imaging of biological samples with high resolution and low phototoxicity.

Gut Stem Cell Secretome Therapy Promotes Durable Neuroprotection and Functional Recovery After Stroke

This study shows that intestinal epithelial stem cell secretome improves gut repair and cognitive outcomes after stroke in male rats but not in female rats, highlighting the need for sex-specific therapies.

COSMOS: integrated vector-pathogen metagenomics for mosquito-borne disease

COSMOS integrates vector-pathogen metagenomics to monitor mosquito-borne diseases, detecting pathogens and mosquito communities in field samples with high accuracy.

Polygenic polymorphisms cooperate to drive expansion of HCMV adaptive NK cell and NK-like T cell populations

This study identifies genetic polymorphisms in NKG2C, CD16a, and HLA-E that influence NKG2C expression and ADCC capacity in HCMV-infected individuals.