A. Mazumder

Aberrant cytoplasmic localization of MLH1 characterizes a sub-clonal breast cancer cell population that seeds recurrence

This study identifies aberrant cytoplasmic MLH1 localization in sub-clonal breast cancer cells as a driver of endocrine resistance, showing it predicts response to CDK4/6 inhibitors.

The displacement of the σ70 finger in initial transcription is highly heterogeneous and promoter-dependent

This study uses smFRET to show that σ70 finger displacement during transcription initiation is heterogeneous and promoter-dependent, affecting gene regulation in bacteria.

Strategic in vitro bioaugmentation of indigenous Actinobacteria isolated from east Kolkata wetlands (Ramsar site) to degrade highly toxic waste engine oil in free and emulsified state

This study shows that Gordonia terrae DSM 43249 can degrade waste engine oil more effectively in microemulsions than in free or coarse emulsions.

Bleaching-resistant, near-continuous single-molecule fluorescence and FRET based on fluorogenic and transient DNA binding

This paper introduces a method to extend single-molecule fluorescence observation times by using fluorogenic DNA binding, enabling near-continuous monitoring for over an hour.

Transient DNA binding to gapped DNA substrates links DNA sequence to the single-molecule kinetics of protein-DNA interactions

The paper introduces Gap-Seq, a single-molecule imaging method that links DNA sequence to protein-DNA interaction kinetics by analyzing transient DNA binding to gapped substrates.

Transcription initiation at a consensus bacterial promoter proceeds via a "bind-unwind-load-and-lock" mechanism

This study reveals the mechanism of promoter unwinding during transcription initiation in bacteria using single-molecule techniques, identifying a 'bind-unwind-load-and-lock' process.

Race specific differences in DNA damage repair dysregulation in breast cancer and association with outcome

The paper identifies race-specific DNA damage repair (DDR) gene dysregulation patterns in African American (AA) ER+ breast cancer patients and links them to poorer survival outcomes.

RNA polymerase clamp conformational dynamics: long-lived states and modulation by crowding, cations, and nonspecific DNA binding

γH2AX in the S Phase After UV Irradiation Corresponds to DNA Replication and Notto the Extent of DNA Damage

This study shows that γH2AX peaks in S phase after UV irradiation due to replication activity, not DNA damage extent, and cells complete replication before arresting in G2.

Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer

This study shows that loss of MLH1, a mismatch repair gene, activates HER2 in ER+/HER2- breast cancer, making these cells sensitive to HER2 inhibitors.

Investigating the Central Dogma of Molecular Biology in the Context of Nuclear Architecture and Cell Cycle

The paper investigates nuclear architecture's role in gene regulation by analyzing CCNA2 expression across the cell cycle using 3D FISH, smFISH, and immunofluorescence.

One-dimensional sliding assists σ70-dependent promoter binding by Escherichia coli RNA polymerase

This study uses single-molecule imaging to show Escherichia coli RNA polymerase σ70 holoenzyme performs one-dimensional sliding on DNA during promoter search, even at high RNAP concentrations.

The RNA polymerase clamp interconverts dynamically among three states and is stabilized in a partly closed state by ppGpp

This study uses single-molecule FRET to show RNA polymerase clamp interconverts among three states, with ppGpp stabilizing the partly closed state during transcription.