B. P. Bowen

DISRUPTION OF THE ENDOGENOUS INDOLE GLUCOSINOLATE PATHWAY IMPACTS THE ARABIDOPSIS THALIANA ROOT EXUDATION PROFILE AND RHIZOBACTERIAL COMMUNITY

This study shows that disrupting the indole glucosinolate pathway in Arabidopsis thaliana alters root exudate profiles and rhizobacterial communities.

Early viral infection of cyanobacteria drives bacterial chemotaxis in the oceans

This study shows that virus-infected cyanobacteria release metabolites that attract heterotrophic bacteria, enhancing nutrient transfer in marine ecosystems.

Reproducible growth of Brachypodium distachyon in fabricated ecosystems (EcoFAB 2.0) reveals that nitrogen form and starvation modulate root exudation

This study uses EcoFAB 2.0 to show nitrogen form and starvation affect Brachypodium root exudation, with specific compounds influencing bacterial and plant growth.

Extensive plant use of exometabolites

The paper uses 13C-tracing to study plant uptake of exometabolites across multiple species, finding that uptake accounts for 23% of the carbon budget in Brachypodium distachyon and that some metabolites promote root growth.

Nutrient and moisture limitation reveal keystone metabolites that link switchgrass rhizosphere metabolome and microbiome dynamics

This study identifies keystone metabolites linking switchgrass rhizosphere metabolome and microbiome dynamics under nutrient and moisture stress.

Characterization and visualization of global metabolomic responses of Brachypodium distachyon to environmental changes

This study characterizes Brachypodium distachyon metabolomic responses to environmental changes using LC-MS/MS and identifies condition-specific metabolic biomarkers.

Tracing carbon metabolism with stable isotope metabolomics reveals the legacy of diverse carbon sources in soil

This study uses stable isotope metabolomics to track 13C from diverse carbon sources in soil over time, showing that carbon source type influences metabolite labeling and microbial activity patterns.

Biofilm Interaction Mapping and Analysis (BIMA): A tool for deconstructing interspecific interactions in co-culture biofilms

The paper introduces BIMA, a method to analyze interspecific interactions in biofilm co-cultures by examining colony morphology, inhibition, invasion, and associated genes/metabolites.

A defined medium based on R2A for cultivation and exometabolite profiling of soil bacteria

The paper develops a defined medium (NLDM) for cultivating soil bacteria and profiling exometabolites, showing it supports growth of 53 diverse isolates and enables metabolite tracking via LC-MS/MS.

Conservation of isolate substrate preferences in mixed communities revealed through ribosomal marker protein profiling

The paper uses ribosomal marker protein profiling to show that isolate substrate preferences are conserved in a synthetic soil community and can be predicted by a weighted-sum model.

Multi-lab EcoFAB study shows highly reproducible physiology and depletion of soil metabolites by a model grass

The paper shows that Brachypodium distachyon consistently responds to soil conditions across labs, depleting soil metabolites and altering root morphology.

Towards measuring growth rates of pathogens during infections by D2O-labeling lipidomics

This paper develops a D2O-labeling lipidomics approach to measure microbial growth rates in infections, finding that deuterium incorporation in lipids can estimate generation times of pathogens like S. aureus.

Linking soil biology and chemistry using bacterial isolate exometabolite profiles

The paper integrates metabolite profiling, sequencing, and exometabolomics to link microbial community structure with environmental chemistry in soil.