Q. Zheng

ScRNA-seq and scATAC-seq reveal that sertoli cell mediate spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia

This study uses scRNA-seq and scATAC-seq to identify stage-specific interactions between Sertoli cell subtypes and germ cells in NOA, revealing potential molecular markers for diagnostic kits.

Bioorthogonal labeling and enrichment of histone monoaminylation reveal its accumulation and regulatory function in cancer cell chromatin

This study develops chemical probes to label and enrich histone monoaminylation, revealing its enrichment in cancer tissues and its role in chromatin architecture regulation.

A series of spontaneously blinking dyes for super-resolution microscopy

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

Capturing Snapshots of Nucleosomal H2A K13/K15 Ubiquitination Mediated by the Monomeric E3 Ligase RNF168

This study determines the cryo-EM structures of RNF168/UbcH5c-Ub/nucleosome complexes to elucidate the mechanism of H2A K13/K15 site-specific ubiquitination.

Spatiotemporal Profiling Unveiling the Cellular Organization Patterns and Local Protumoral Immune Microenvironment Remodeling in Early Lung Adenocarcinoma Progression

The paper uses spatial transcriptomics and single-cell RNA sequencing to identify cellular organization patterns and immune microenvironment changes in early lung adenocarcinoma progression.

Convergent alterations in the tumor microenvironment of MYC-driven human and murine prostate cancer

This study identifies MYC signaling activation as a common driver of tumor microenvironment alterations in human and murine prostate cancer through single-cell RNA-sequencing and comparative analysis.

Structure basis of the phytoplasma effector SAP05 recognition specificities to plant Rpn10 in ubiquitin-independent protein degradation

The paper determines the crystal structure of phytoplasma effector SAP05 bound to plant Rpn10, revealing a ubiquitin-independent degradation mechanism and conserved interaction patterns across species.

Strain-release alkylation of Asp12 enables mutant selective GTP-state targeting of K-Ras(G12D)

This paper develops covalent inhibitors that target the K-Ras(G12D) mutation by crosslinking aspartate-12 in both GDP- and GTP-states, effectively suppressing cancer cell proliferation and tumor growth.

Structural Visualization of HECT-type E3 ligase Ufd4 Accepting and Transferring Ubiquitin to Form K29/K48-branched Polyubiquitination

This study determines the structural mechanism of HECT-type E3 ligase Ufd4 in catalyzing K29/K48-branched polyubiquitination by capturing structural snapshots of Ub transfer cascades.

Chromogenic detection of telomere lengths in situ aids the identification of precancerous lesions in the prostate

This paper introduces a chromogenic in situ hybridization method to measure telomere lengths in prostate tissues, aiding in the identification of precancerous lesions.

MYC-driven increases in mitochondrial DNA copy number occur early and persist throughout prostatic cancer progression

The paper develops a method to spatially quantify mitochondrial DNA copy number in cancer tissues and finds increased mtDNAcn in prostate cancer progression, linked to MYC activity.

Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine

This study develops a bivalent vaccine using lineage-mosaic and mutation-patched spike proteins that elicit broad-spectrum antibodies and protect against multiple SARS-CoV-2 variants in hamsters.

Conventional dendritic cells 2, the targeted antigen-presenting-cell, induces enhanced type 1 immune responses in mice immunized with CVC1302 in oil formulation

This study shows that CVC1302 in oil formulation enhances Th1 immune responses by targeting antigen and inflammatory monocytes to lymph node inter-follicular regions and promoting IL-12 production by cDC2.

Integrated global analysis in spider-flowers illuminates features underlying the evolution and maintenance of C4 photosynthesis

This study analyzes the genome and multi-omics data of a C4 plant to uncover evolutionary mechanisms behind C4 photosynthesis development.

Harnessing diversity and antagonism within the pig skin microbiota to identify novel mediators of colonization resistance to methicillin-resistant Staphylococcus aureus

The paper identifies novel microbiota-mediated mechanisms of colonization resistance to MRSA in pig skin using a swine model and isolates.

Extensive plant use of exometabolites

The paper uses 13C-tracing to study plant uptake of exometabolites across multiple species, finding that uptake accounts for 23% of the carbon budget in Brachypodium distachyon and that some metabolites promote root growth.

Ploidy variation and spontaneous haploid-diploid switching of Candida glabrata clinical isolates

This study reports ploidy variation and spontaneous haploid-diploid switching in 500 clinical Candida glabrata isolates, revealing differences in morphology, antifungal susceptibility, virulence, and gene expression.

A potent synthetic nanobody with broad-spectrum activity neutralizes SARS-Cov-2 virus and Omicron variant through a unique binding mode

This paper reports a synthetic nanobody, C5G2, that neutralizes SARS-CoV-2 and Omicron variants with high potency through a unique binding mechanism.

Lack of universal mutational biases in a fungal phylum

The paper analyzes mutational biases across 537 fungal strains from Ascomycota, finding variation in A|T and G|C substitution patterns and showing that genome composition is influenced by both mutational bias and selection.

KRAS is vulnerable to reversible switch-II pocket engagement in cells

The paper investigates the accessibility of the switch-II pocket in various KRAS mutants using NMR and a cellular engagement assay, showing it is accessible with non-covalent ligands regardless of GDP state.

Single-cell atlas of epithelial and stromal cell heterogeneity by lobe and strain in the mouse prostate

The paper constructs a single-cell atlas of mouse prostate cell types across strains and lobes, revealing strain- and lobe-specific transcriptomic differences in epithelial cells and strain-lobespecific fibroblast subtypes.

Knockout of OsSWEET15 impaired rice embryo formation and seed-setting

This study shows that knocking out OsSWEET15 in rice reduces fertility by impairing embryo formation and seed-setting, with similar effects observed in OsSWEET11 mutants.

Molecular basis of broad neutralization against SARS-CoV-2 variants including Omicron by a human antibody

A human antibody, VacW-209, broadly neutralizes SARS-CoV-2 variants including Omicron by targeting a conserved epitope, highlighting the importance of such antibodies in pandemic control.

PPAR-delta acts as a metabolic master checkpoint for metastasis in pancreatic cancer

This study shows PPAR-delta drives metabolic reprogramming in pancreatic cancer, promoting metastasis, and its inhibition reduces invasiveness.

Ghrelin proteolysis by insulin-degrading enzyme

The paper investigates ghrelin proteolysis by insulin-degrading enzyme, identifying cleavage sites and developing a fluorogenic substrate for future studies.

Glycine-Rich RNA-Binding Protein 7 interacts with and potentiates effector-induced immunity by Gpa2 and Rx1 based on an intact RNA Recognition Motif

This study identifies NbGRP7 as a novel RNA-binding protein that enhances immunity by interacting with Gpa2 and Rx1, regulating their function through an intact RNA recognition motif.

Structural Insights Into the Initiation and Elongation of Ubiquitination by Ubr1

This study reports cryo-EM structures of Ubr1 complexes to elucidate the mechanism of ubiquitination initiation and elongation using a single E2 enzyme.

Single cell transcriptome analysis of decidua macrophages in normal and recurrent spontaneous abortion patients

The paper profiles single-cell transcriptomes of decidual macrophages in normal and recurrent spontaneous abortion patients, identifying distinct polarization states and potential therapeutic targets.

A H3K9me2-Binding Protein AGDP3 Limits DNA Methylation and Transcriptional Gene Silencing in Arabidopsis

This study identifies AGDP3 as a protein that binds H3K9me2 and facilitates ROS1-mediated DNA demethylation in Arabidopsis, revealing a mechanism linking histone modification to gene regulation.

Mechanistic insight into substrate processing and allosteric inhibition of human p97

This study presents cryo-EM structures of human p97 complex with and without inhibitor NMS-873, revealing conformational changes and allosteric inhibition mechanisms.