J. H. D. Cate

The Helix-Loop-Helix motif of human EIF3A regulates translation of proliferative cellular mRNAs

This study shows that the EIF3A HLH motif regulates translation of oncogenic mRNAs like MYC, affecting cell proliferation and response to anti-cancer drugs.

Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells

This study investigates chromosome loss in CRISPR-Cas9-engineered T cells and shows a modified manufacturing process reduces it while preserving editing efficacy.

Aminobenzoic acid derivatives obstruct induced fit in the catalytic center of the ribosome

This study reports cryo-EM structures of E. coli ribosomes with aminobenzoic acid derivatives, revealing how steric blocking and proton wire disruption affect amide bond formation efficiency.

Candidatus Nealsonbacteria (OD1) are biomass recycling ectosymbionts of methanogenic archaea in a stable benzene-degrading enrichment culture

The paper identifies Ca. Nealsonbacteria DGGOD1a as a biomass recycling ectosymbiont of methanogenic archaea in a benzene-degrading consortium.

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

This paper introduces a method for isolating rice protoplasts from calli with pause points, enabling flexible transfection and genome editing assays.

Structure of the Bacterial Ribosome at 2 A Resolution

This paper presents a 2.0 Å resolution model of the E. coli 70S ribosome using cryo-EM, revealing detailed chemical interactions, modifications, and solvation.

Selective inhibition of human translation by a drug-like compound that traps terminated protein nascent chains on the ribosome

The paper shows PF846 inhibits translation by trapping nascent chains on the ribosome, revealing a new mechanism for targeting protein synthesis.

Defects in the assembly of ribosomes selected for β-amino acid incorporation

This study investigates a mutant ribosome, P7A7, evolved for β-amino acid incorporation, revealing defects in its assembly that reduce in vitro activity.

Differences in the path to exit the ribosome across the three domains of life

The paper compares ribosome exit tunnel structures across bacteria, archaea, and eukarya to understand geometric and sequence conservation patterns and their implications for translation dynamics.

Modulating long-range energetics via helix stabilization: a case study using T4 lysozyme

This study investigates long-range energetic coupling in T4 lysozyme by analyzing a circularly permuted variant, showing that helix stabilization enhances subdomain stability through salt bridge interactions.

Monolayer-crystal streptavidin support films provide an internal standard of cryo-EM image quality

The paper shows that image quality of cryo-EM samples can be predicted by the quality of streptavidin monolayer crystals, enabling better data selection.