R. Subramanian

Discovery and Synthesis of GS-7682, a Novel Prodrug of a 4'-CN-4-Aza-7,9-Dideazaadenosine C-Nucleoside with Broad-Spectrum Potency Against Pneumo- and Picornaviruses and Efficacy in RSV-Infected African Green Monkeys.

This paper describes the discovery and synthesis of GS-7682, a prodrug with broad antiviral activity against RSV and other viruses, showing efficacy in RSV-infected monkeys.

Antigenic cartography using hamster sera identifies SARS-CoV-2 JN.1 evasion seen in human XBB.1.5 booster sera

The paper uses hamster sera to map antigenic differences among SARS-CoV-2 variants, showing JN.1 has significant antigenic divergence from XBB.1.5.

Molecular determinants of Neu5Ac binding to tripartite ATP independent periplasmic (TRAP) transporter

The paper reports the structures of SiaQM from Fusobacterium nucleatum with and without Neu5Ac, revealing interactions and transport mechanisms.

Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast

This study reveals the architecture and flexibility of native kinetochores using structural analyses of purified kinetochores from thermophilic yeast.

Torques within and outside the human spindle balance twist at anaphase

The paper investigates how molecular torques balance spindle twist during anaphase in human cells, identifying factors that increase or decrease twist.

Structural and biophysical analysis of a Haemophilus influenzae tripartite ATP-independent periplasmic (TRAP) transporter

This study reports the cryo-EM structure of a TRAP transporter from Haemophilus influenzae, revealing its dimeric architecture, Na+ sites, and substrate binding site, supported by mutagenic and functional data.

GRK2 Kinases in the Primary Cilium Initiate SMOOTHENED-PKA Signaling in the Hedgehog Cascade

This study identifies GRK2-mediated phosphorylation of SMO in the primary cilium as a critical step initiating SMO-PKA signaling in the Hedgehog pathway.

Discovery of GS-5245 (Obeldesivir), an Oral Prodrug of Nucleoside GS-441524 that Exhibits Antiviral Efficacy in SARS-CoV-2 Infected African Green Monkeys

FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity

FALCON systematically analyzes 61 free fatty acids to identify lipotoxic effects and a novel mediator of lipotoxicity, CMIP, in human pancreatic beta cells.

Biochemical characterization of a GDP-mannose transporter from Chaetomium thermophilum

The paper characterizes CtVrg4, a GDP-mannose transporter from Chaetomium thermophilum, showing enhanced thermal stability and phosphatidylinositol lipid binding.

Variability of Phenylalanine side chain conformations facilitates promiscuity of Fatty acid binding in Cockroach milk proteins.

This study investigates fatty acid binding specificity and stability of Lili-Mips proteins in the cockroach Diploptera punctata, showing similar affinities for different fatty acids and pH-dependent stability.

Spatially controlled microtubule nucleation and organization from crosslinker MAP65 condensates

This study shows that MAP65-1 forms phase-separated condensates that nucleate microtubule bundles in plant cells, suggesting a universal mechanism for microtubule organization.

A Dimer between Monomers and Hexamers - Oligomeric Variations in Glucosamine 6-Phosphate Deaminase Family

This paper identifies a dimeric form of NagB in Gram-negative bacteria and characterizes its structural basis, suggesting it as an evolutionary link between monomeric and hexameric forms.

Post-vaccination Omicron infections induce broader immunity across antigenic space than prototype mRNA COVID-19 booster vaccination or primary infection

The paper compares antibody responses to different SARS-CoV-2 variants after vaccination and infection, finding that post-vaccination Omicron infection induces broader immunity than triple vaccination alone.

Cytoskeletal regulation of a transcription factor by DNA mimicry

The paper reveals DNA mimicry by Kif7 as a mechanism for recruiting Gli transcription factors to microtubules, showing it can be used to inhibit Gli localization in cancer.

Therapeutic efficacy of an oral nucleoside analog of remdesivir against SARS-CoV-2 pathogenesis in mice.

This paper evaluates the therapeutic efficacy of GS-621763, an oral prodrug of remdesivir, against SARS-CoV-2 in mice, showing reduced viral load and improved lung function.

Structural heterogeneity in biliverdin modulates spectral properties of Sandercyanin fluorescent protein

This paper reports the spectral properties and crystal structures of engineered monomeric Sandercyanin-BLA complexes, revealing how structural heterogeneity in biliverdin modulates fluorescence.

Motor guidance by long-range communication through the microtubule highway

The paper shows that microtubules can mediate long-range coupling between motors, affecting their movement and mechanochemistry without direct interaction.

Atomic Force Microscopy Reveals Distinct Protofilament-scale Structural Dynamics in Depolymerizing Microtubule Arrays

This paper uses AFM to visualize microtubule array dynamics at protofilament resolution, revealing distinct depolymerase activities in MCAK and Kip3p.

Differential Regulation of Single Microtubules and Bundles by a Three-Protein Module

The paper investigates how a three-protein module differentially regulates single microtubules and bundles, revealing mechanisms that create distinct dynamic properties.

The Arabidopsis gene RGO mediates cytokinin responses and increases seed yield

RGO, a novel gene from Arabidopsis, is essential for plant development, mediates CK signaling and increases seed yield in Arabidopsis and rapeseed when overexpressed.

Breaking a Strong Amide Bond: Structure and Properties of Dimethylformamidase.

This paper describes the structure and properties of Dimethylformamidase (DMFase) from Paracoccus, revealing new protein folds and a unique mononuclear iron active site that enables its function in harsh conditions.

Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A

The paper shows how the geometry of antiparallel microtubule bundles affects sliding and stalling by PRC1 and Kif4A through changes in overlap length and final overlap width.