D. Agard

Defining the constituents and functions of a lipid-based jumbo phage compartment

The paper identifies a lipid-based compartment in early phage infection that protects phage DNA and supports early transcription and translation.

Poc1 is a basal body inner junction protein that promotes triplet microtubule integrity and interconnections

This study identifies Poc1 as a basal body protein that stabilizes triplet microtubules and interconnections, enhancing resistance to ciliary forces.

AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction

AreTomo automates motion-corrected cryo-electron tomographic alignment and reconstruction, enabling high-resolution subtomogram averaging and real-time feedback during data collection.

Structural features of mTORC2 that control substrate-specific activities.

The paper determines the structure of mTORC2 and shows how substrate-specific interactions and conformational changes regulate its activity.

Electron Cryo-Tomography Structure of Axonemal Doublet Microtubule from Tetrahymena thermophila

The paper uses electron cryo-tomography to study axoneme DMT structures from Tetrahymena thermophila, revealing MIP interactions and their roles in DMT stability and function.

Cryo-EM reveals the dynamic interplay between mitochondrial Hsp90 and SdhB folding intermediates

The paper uses cryo-EM to reveal how TRAP1 interacts with SdhB folding intermediates, showing client binding induces structural changes in TRAP1 and provides insights into its role in mitochondrial function.

Cryo-EM structures reveal a multistep mechanism of Hsp90 activation by co-chaperone Aha1

The paper determines cryo-EM structures of Hsp90:Aha1 complexes in multiple states to reveal a multistep activation mechanism where Aha1 accelerates ATP hydrolysis and induces conformational changes in Hsp90.

XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions

This study investigates whether XMAP215 and γ-tubulin synergize in microtubule nucleation and finds they act additively, not synergistically, in purified solutions.

The interplay between Bag-1, Hsp70, and Hsp90 reveals that inhibiting Hsp70 rebinding is essential for Glucocorticoid Receptor activity

This paper investigates the interplay between Bag-1, Hsp70, and Hsp90 in GR reactivation, finding that inhibiting Hsp70 re-binding is critical for GR activity, with Hsp90 playing a unique role in protecting GR from re-inactivation.

Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy

This paper introduces amino and PEG-amino functionalized graphene oxide grids to improve sample concentration, distribution, and protection for cryo-EM.

General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM

This paper develops covalently linked graphene oxide affinity grids for cryo-EM, enabling high-resolution reconstructions by preventing particle denaturation and aggregation.

Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

This paper introduces micro-expansion joints to reduce lamella bending during cryo-FIB-milling, improving structural studies of biological samples.

Electron Cryo-Tomography Provides Insight into Procentriole Architecture and Assembly Mechanism

The paper uses electron cryo-tomography to reveal the structure and assembly mechanism of procentrioles in Chlamydomonas reinhardtii, identifying key components and intermediates.

A nucleus-like compartment shields bacteriophage DNA from CRISPR-Cas and restriction nucleases

This study identifies a nucleus-like compartment in a jumbo phage that protects its DNA from CRISPR-Cas and restriction nucleases, but leaves its mRNA vulnerable to Cas13a.