Author

D. Barford

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 6 Cell Biology· 3 Cancer Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 9 Research Focus:protein structure prediction· 5 Assay:cryo-EM· 4 Organism:human· 4 Research Focus:cell signaling & trafficking· 3 Research Focus:chromatin & epigenetic regulation· 3 Research Focus:protein folding & dynamics· 2 Assay:protein structure· 2

Frequent Co-Authors

J. Yang· 7 shared Z. Zhang· 6 shared S. H. McLaughlin· 4 shared S. Yatskevich· 3 shared T. Tischer· 3 shared D. Bellini· 2 shared E. S. Fischer· 2 shared T. Dendooven· 2 shared

Activity by Year

2020-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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7 shared papersBiochemistry

J. Yang

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10 papers

Recent·Most cited

New structural features of the APC/C revealed by high resolution cryo-EM structures of apo-APC/C and the APC/C CDH1:EMI1 complex

A. Hoefler, J. Yu, J. Yang, Z. Zhang, L. Chang, S. H. McLaughlin, G. W. Grime, E. F. Garman, A. Boland, D. Barford

This study uses cryo-EM to determine high-resolution structures of apo-APC/C and APC/C CDH1:EMI1, revealing new structural features and regulatory mechanisms.

Nature Communications · Nov 21, 2024Peer-reviewedCell Biology

Development of D-box peptides to inhibit the Anaphase Promoting Complex/Cyclosome

R. Eapen, C. Okoye, C. Stubbs, M. Schimpl, T. Tischer, E. J. Fisher, M. Zacharopoulou, F. Ferrer, D. Barford, D. Spring, C. Lindon, C. Phillips, L. S. Itzhaki

This study develops D-box peptides that inhibit APC/C by binding Cdc20, showing they are more potent than Apcin and can drive protein degradation.

bioRxiv · Apr 30, 2024Biochemistry

Molecular mechanism of Mad2 conformational conversion promoted by the Mad2-interaction motif of Cdc20

C. W. H. Yu, E. S. Fischer, J. G. Greener, J. Yang, Z. Zhang, S. M. V. Freund, D. Barford

This study elucidates how the Cdc20MIM induces Mad2 conformational conversion by transiently exposing a binding site and facilitating structural changes.

bioRxiv · Mar 3, 2024Biochemistry

Structure of the human outer kinetochore KMN network complex

S. Yatskevich, J. Yang, D. Bellini, Z. Zhang, D. Barford

The paper determines the high-resolution cryo-EM structure of the human KMN network, revealing its subunit organization and phosphorylation-dependent regulation.

bioRxiv · Aug 7, 2023Cell Biology

Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere

T. Dendooven, Z. Zhang, J. Yang, S. H. McLaughlin, J. Schwab, S. H. W. Scheres, S. Yatskevich, D. Barford

The paper determines the cryo-EM structure of the complete inner kinetochore of budding yeast, revealing its architecture and DNA interactions.

bioRxiv · Dec 12, 2022Biochemistry

Structural basis for CDC42 and RAC activation by the dual specificity GEF DOCK10

D. Fan, J. Yang, N. Cronin, D. Barford

This paper determines the crystal structure of DOCK10DHR2, revealing its dual specificity for CDC42 and RAC, and a unique 2:1 stoichiometry in complex with RAC.

bioRxiv · Jun 15, 2022 · PreprintBiochemistry

Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly

E. S. Fischer, C. W. Yu, J. F. Hevler, S. H. McLaughlin, S. L. Maslen, A. J. Heck, S. M. Freund, D. Barford

This study investigates how Mps1 phosphorylation of Mad1 facilitates the assembly of the mitotic checkpoint complex by promoting interactions between Mad1, Cdc20, and Bub1.

bioRxiv · May 16, 2022Biochemistry

Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome

S. Yatskevich, K. W. Muir, D. Bellini, Z. Zhang, J. Yang, T. Tischer, M. Predin, T. Dendooven, S. H. McLaughlin, D. Barford

The paper describes the cryo-EM structure of the human inner kinetochore CCAN complex bound to a CENP-A nucleosome, revealing how it grips linker DNA for chromosome segregation.

bioRxiv · Jan 7, 2022Biochemistry

Germline Missense Variants in CDC20 Result in Aberrant Mitotic Progression and Familial Cancer

O. J. Chen, E. Castellsague, M. Moustafa-Kamal, J. Nadaf, B. Rivera, S. Fahiminiya, Y. Wang, I. Gamache, C. Pacifico, L. Jiang, J. Carrot-Zhang, L. Witkowski, A. M. Berghuis, S. Schoenberger, D. Schneider, , J. G. Teodoro

The paper identifies CDC20 missense variants linked to familial cancer, showing they impair SAC binding and promote mitotic slippage.

bioRxiv · Sep 13, 2021Cancer Biology

Negative feedback between the APC/C and Cep152 at the centrosome regulates mitotic spindle assembly

T. Tischer, J. Yang, D. Barford

The paper shows that APC/C and Cep152 interact at centrosomes during mitosis, with APC/C regulating Cep152 levels to control spindle assembly and chromosome segregation.

bioRxiv · Mar 23, 2020Cell Biology