S. H. McLaughlin

Positive cooperativity enhances coupling of a second G protein to a GPCR dimer

The paper shows that a second G protein couples with higher affinity to a GPCR dimer than the first, due to conformational stabilization by the first G protein.

New structural features of the APC/C revealed by high resolution cryo-EM structures of apo-APC/C and the APC/C CDH1:EMI1 complex

This study uses cryo-EM to determine high-resolution structures of apo-APC/C and APC/C CDH1:EMI1, revealing new structural features and regulatory mechanisms.

Disease-specific tau filaments assemble via polymorphic intermediates

This study uses cryo-EM to analyze intermediate structures in tau filament assembly, revealing shared and condition-dependent intermediates in Alzheimer's and CTE pathogenesis.

Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere

The paper determines the cryo-EM structure of the complete inner kinetochore of budding yeast, revealing its architecture and DNA interactions.

Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly

This study investigates how Mps1 phosphorylation of Mad1 facilitates the assembly of the mitotic checkpoint complex by promoting interactions between Mad1, Cdc20, and Bub1.

Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome

The paper describes the cryo-EM structure of the human inner kinetochore CCAN complex bound to a CENP-A nucleosome, revealing how it grips linker DNA for chromosome segregation.

The bacterial actin-like cell division protein FtsA forms curved antiparallel double filaments upon binding of FtsN

This study shows that FtsA forms curved antiparallel double filaments when bound to FtsN, similar to MreB, and suggests these structures orient septal PG synthesis during bacterial division.

Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace

This study investigates the interaction between DEPTOR and mTORC1, revealing DEPTOR as a partial inhibitor that is more effective against activated mTORC1, with mutual antagonism mediated by phosphorylation and structural interactions.

SARS-CoV-2 spike protein arrested in the closed state induces potent neutralizing responses

This study evaluates closed-state SARS-CoV-2 spike protein immunization in mice, finding it induces potent neutralizing antibodies and T-cell responses, with unique binding properties compared to standard S protein constructs.

Structure of the tripartite motif of KAP1/TRIM28 identifies molecular interfaces required for transcriptional silencing of retrotransposons

This study identifies molecular interfaces in KAP1's RBCC motif critical for self-association and KRAB binding, essential for retrotransposon silencing.

An oligomeric state-dependent switch in FICD regulates AMPylation and deAMPylation of the chaperone BiP

This paper identifies how FICD switches between AMPylation and deAMPylation of BiP by changing its oligomeric state, regulated by ATP/ADP levels.

An equilibrium binding model for CpG DNA-dependent dimerization of Toll-like receptor 9

This study develops a theoretical equilibrium binding model to explain how CpG DNA induces TLR9 dimerization, using experimental data from light scattering and fluorescence polarization assays.

Structural analysis of the interaction between the bacterial cell division proteins FtsQ and FtsB

This study investigates the interaction between FtsQ and FtsB in E. coli, revealing key residues and structural details critical for cell division.

RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs

The paper reconstitutes microtubule minus end-directed mRNA transport using purified components and shows RNA is required for dynein activation.