D. J. Doorduijn

Colistin resistance mutations in phoQ sensitize Klebsiella pneumoniae to IgM-mediated complement killing

This study shows that colistin resistance mutations in K. pneumoniae increase sensitivity to IgM-mediated complement killing via PhoQ activation.

Soluble MAC is primarily released from MAC-resistant bacteria that potently convert complement component C5

The paper investigates how soluble MAC (sMAC) is formed during bacterial infections, finding that MAC-resistant bacteria convert C5 into C5b more efficiently and release sMAC, which can lyse bystander erythrocytes.

Polymerization of C9 enhances bacterial cell envelope damage and killing by membrane attack complex pores

This study shows that C9 polymerization enhances MAC pore-mediated bacterial killing and is impaired in complement-resistant strains.

In vitro evolution of colistin resistance in the Klebsiella pneumoniae complex follows multiple evolutionary trajectories with variable effects on fitness and virulence characteristics.

This study shows that Klebsiella pneumoniae complex strains can develop colistin resistance through multiple evolutionary paths, with variable effects on fitness and virulence.

Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7

This study shows that direct anchoring of C5b-7 is essential for MAC pore formation and bacterial killing, and bacteria can evade this by interfering with anchoring.