D. L. Lafontaine

Detecting Material State Changes in the Nucleolus by Label-free Digital Holographic Microscopy

This study uses digital holographic microscopy to detect and quantify nucleolar changes in human cells, identifying material state alterations without staining.

Parvovirus infection affects host cell nucleolar organization and ribosome biogenesis

This study investigates how canine parvovirus infection alters nucleolar structure and ribosome biogenesis using advanced imaging and biochemical techniques.

Glutamine deficiency in solid tumors confers resistance to ribosomal RNA synthesis inhibitors

The paper shows that glutamine deficiency in cancer cells prevents p53 activation by rRNA synthesis inhibitors, promoting survival during nutrient stress.

Systematic mapping of small nucleolar RNA targets in human cells

The paper identifies RNA targets of box C/D snoRNAs in human cells using CLASH and FLASH techniques, revealing novel interactions with rRNA, snoRNAs, and mRNAs.

Nopp140-chaperoned 2'-O-methylation of small nuclear RNAs in Cajal bodies ensures splicing fidelity

This study identifies CBs as the site of snRNA modification by scaRNPs, showing that Nopp140 and CK2 are critical for splicing fidelity and that CK2 inhibitors may affect splicing in diseases.

The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans

This study identifies NSUN-1 as an enzyme that methylates rRNA at position C2982 in C. elegans, showing that its depletion affects healthspan, oogenesis, and cuticle integrity.