Author

H. S. Overkleeft

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Bioengineering· 1 Immunology· 1 Physiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:protein folding & dynamics· 2 Assay:protein structure· 2 Organism:bacteria (other)· 1 Research Focus:cell type annotation· 1 Research Focus:host-pathogen & immune response· 1 Organism:human· 1 Approach:machine learning (classical)· 1

Frequent Co-Authors

F. Ben Bdira· 2 shared M. Ubbink· 2 shared A. Cramer-Blok· 1 shared A. Kaptein· 1 shared A. N. Volkov· 1 shared A. S. B. Kampstra· 1 shared B. I. Florea· 1 shared C. A. Waudby· 1 shared

Activity by Year

2019-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 10 mutual co-author links

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2 shared papersBiochemistry

F. Ben Bdira

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Author profile →

5 papers

Recent·Most cited

Combinatorial assembly and design of enzymes

R. Lipsh-Sokolik, O. Khersonsky, S. P. Schroder, C. de Boer, S. Y. Hoch, G. J. Davies, H. S. Overkleeft, S. J. Fleishman

The paper introduces CADENZ, a machine-learning strategy for designing structurally diverse enzymes, achieving a tenfold improved hit rate and over 10,000 active enzymes.

bioRxiv · Sep 17, 2022Bioengineering

Tuning the transglycosylation reaction of a GH11 xylanase by a delicate enhancement of its thumb flexibility

K. Marneth, H. van den Elst, A. Cramer-Blok, J. D. C. Codee, H. S. Overkleeft, H. M. F. G. Aerts, M. Ubbink, F. Ben Bdira

This study modifies a GH11 xylanase to enhance transglycosylation by increasing thumb flexibility through a point mutation, altering its enzymatic balance and dynamics.

bioRxiv · Dec 19, 2020Biochemistry

Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis via SYK activation

T. van der Wel, R. Hilhorst, H. den Dulk, T. van den Hooven, N. M. Prins, J. A. P. M. Wijnakker, B. I. Florea, E. B. Lenselink, G. J. P. van Westen, R. Ruijtenbeek, H. S. Overkleeft, A. Kaptein, T. Barf, M. van der Stelt

The paper uses a chemical genetics strategy to study FES kinase target engagement in human cells, revealing its role in neutrophil phagocytosis via SYK activation.

bioRxiv · Nov 13, 2019Physiology

Catalysis by a rigid enzyme

F. Ben Bdira, C. A. Waudby, A. N. Volkov, S. Schroder, E. AB, J. Codee, H. S. Overkleeft, H. Aerts, H. van Ingen, M. Ubbink

The paper shows that the rigid xylanase enzyme uses substrate flexibility to facilitate catalysis without significant structural changes.

bioRxiv · Oct 24, 2019 · PreprintBiochemistry

Bioorthogonal antigens allow the unbiased study of antigen processing and presentation

C. Araman, L. Pieper-Pournara, T. van Leeuwen, A. S. B. Kampstra, T. Bakkum, M. H. S. Marqvorsen, C. Nascimento, W. van der Wulp, M. G. J. M. Groenewold, M. G. M. Camps, H. S. Overkleeft, F. Ossendorp, R. Toes, S. I. van Kasteren

The paper explores how bioorthogonal antigens can be used to study antigen processing and presentation without bias.

bioRxiv · Jun 4, 2019 · PreprintImmunology