S. J. Fleishman

Preclinical Development of a Stabilized RH5 Virus-Like Particle Vaccine that Induces Improved Anti-Malarial Antibodies

This paper develops a stabilized RH5 VLP vaccine that induces improved anti-malarial antibodies compared to a soluble protein vaccine in preclinical studies.

Stable mammalian serum albumins designed for bacterial expression

This paper designs stable mammalian serum albumins for bacterial expression, showing improved thermostability and ligand binding properties.

One-shot design elevates functional expression levels of a voltage-gated potassium channel

The paper introduces mPROSS, an algorithm that improves the functional expression of membrane proteins through one-shot design, achieving up to 14-fold increase in current densities without altering single-channel activity.

Designed active-site library reveals thousands of functional GFP variants

The paper designs a library of functional GFP variants using a machine-learning approach, recovering over 16,000 unique sequences with improved thermostability and fluorescence properties.

Combinatorial assembly and design of enzymes

The paper introduces CADENZ, a machine-learning strategy for designing structurally diverse enzymes, achieving a tenfold improved hit rate and over 10,000 active enzymes.

Reliable energy-based antibody humanization and stabilization

The paper introduces CUMAb, a computational method for humanizing antibodies that improves stability and binding by systematically grafting CDRs onto human frameworks and ranking designs using Rosetta simulations.

Designed high-redox potential laccases exhibit high functional diversity

The paper designs high-redox potential laccases with improved stability and functional diversity through stabilization and active-site engineering.

Protein Quaternary Structures in Solution are a Mixture of Multiple forms

The paper assesses the oligomeric states of 17 bacterial proteins using multiple techniques, finding that most exist in multiple forms and that AlphaFold Multimer better predicts their quaternary structures than existing databases.

Stable and functionally diverse versatile peroxidases by computational design directly from sequence

The paper designs stable and functionally diverse versatile peroxidases using computational methods, showing improved expression and environmental tolerance compared to wild-type enzymes.

Stabilization of the SARS-CoV-2 Receptor Binding Domain by Protein Core Redesign and Deep Mutational Scanning

The paper stabilizes the SARS-CoV-2 RBD using deep mutational scanning and computational design, resulting in a more thermally stable and proteolytically resistant variant.

Assessing and enhancing foldability in designed proteins

This study develops methods to assess and improve foldability in designed proteins, significantly enhancing catalytic efficiency and stability.

The neutralization potency of anti-SARS-CoV-2 therapeutic human monoclonal antibodies is retained against novel viral variants

This study evaluates the neutralization potency of four SARS-CoV-2 mAbs against spike mutations and two variants of concern, finding that MD65 and BL6 retain effectiveness against B.1.1.7 and B.1.351.

A computationally designed fluorescent biosensor for D-serine

The paper designs a fluorescent biosensor for D-serine by engineering an SBP with improved affinity, specificity, and thermostability, enabling its use in measuring D-serine concentrations in brain slices.

Coronacept - a potent immunoadhesin against SARS-CoV-2

The paper describes the design of a modified human ACE2 immunoadhesin with enhanced affinity for SARS-CoV-2 spike protein, showing its potential as an antiviral agent.

A lipophilicity-based energy function for membrane-protein modelling and design

This paper introduces a lipophilicity-based energy function for membrane-protein modelling and design, showing improved performance in benchmarks compared to previous methods.