Cell-surface tethered promiscuous biotinylators enable small-scale surface proteomics of human exosomes
L. L. Kirkemo, S. Elledge, J. Yang, J. R. Byrnes, J. E. Glasgow, R. Blelloch, J. A. Wells
This paper introduces two methods for rapid, small-scale cell surface biotinylation using tethered enzymes, enabling surface proteome analysis of cells and exosomes with high coverage and quantification of 1,020 surface proteins.
bioRxiv · Sep 22, 2021Biochemistry